Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 16390..16915 Replicon plasmid unnamed1
Accession NZ_CP062998
Organism Klebsiella pneumoniae strain CriePir197

Toxin (Protein)


Gene name ccdB Uniprot ID A0A7H9GHC5
Locus tag GJJ12_RS26040 Protein ID WP_009309918.1
Coordinates 16390..16695 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag GJJ12_RS26045 Protein ID WP_006788213.1
Coordinates 16697..16915 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJJ12_RS26000 11578..11820 - 243 Protein_12 phosphodiesterase MrkJ -
GJJ12_RS26005 11969..12949 - 981 WP_023279681.1 IS5-like element ISKpn26 family transposase -
GJJ12_RS26010 13052..13165 + 114 WP_014343462.1 hypothetical protein -
GJJ12_RS26015 13294..13551 + 258 WP_009310077.1 hypothetical protein -
GJJ12_RS26020 13609..14388 - 780 WP_023287113.1 site-specific integrase -
GJJ12_RS26025 14586..15602 - 1017 WP_009309921.1 hypothetical protein -
GJJ12_RS26030 15636..15971 - 336 WP_009309920.1 hypothetical protein -
GJJ12_RS26035 16021..16161 - 141 WP_162898808.1 hypothetical protein -
GJJ12_RS26040 16390..16695 - 306 WP_009309918.1 type II toxin-antitoxin system toxin CcdB Toxin
GJJ12_RS26045 16697..16915 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
GJJ12_RS26050 17512..17742 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
GJJ12_RS26055 17739..18155 + 417 WP_020804312.1 type II toxin-antitoxin system VapC family toxin -
GJJ12_RS26060 18196..19074 - 879 WP_009309916.1 restriction endonuclease -
GJJ12_RS26065 19553..20578 + 1026 WP_001101446.1 IS110 family transposase -
GJJ12_RS26070 20897..21865 - 969 WP_077254959.1 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - mrkF / mrkJ 1..114497 114497


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11571.24 Da        Isoelectric Point: 6.4661

>T177441 WP_009309918.1 NZ_CP062998:c16695-16390 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVVPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T177441 NZ_CP083492:c3082821-3082648 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT177441 WP_006788213.1 NZ_CP062998:c16915-16697 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT177441 NZ_CP083492:c3083145-3082849 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H9GHC5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References