Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5188646..5189271 Replicon chromosome
Accession NZ_CP062997
Organism Klebsiella pneumoniae strain CriePir197

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag GJJ12_RS25555 Protein ID WP_002882817.1
Coordinates 5188646..5189029 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag GJJ12_RS25560 Protein ID WP_004150355.1
Coordinates 5189029..5189271 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJJ12_RS25540 5186012..5186914 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
GJJ12_RS25545 5186911..5187546 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
GJJ12_RS25550 5187543..5188472 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
GJJ12_RS25555 5188646..5189029 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
GJJ12_RS25560 5189029..5189271 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
GJJ12_RS25565 5189476..5190393 + 918 WP_004178029.1 alpha/beta hydrolase -
GJJ12_RS25570 5190407..5191348 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
GJJ12_RS25575 5191393..5191830 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
GJJ12_RS25580 5191827..5192687 - 861 WP_002882807.1 virulence factor BrkB family protein -
GJJ12_RS25585 5192681..5193280 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T177440 WP_002882817.1 NZ_CP062997:c5189029-5188646 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T177440 NZ_CP083492:c2979876-2979526 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGTCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT177440 WP_004150355.1 NZ_CP062997:c5189271-5189029 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT177440 NZ_CP083492:c2980121-2979870 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References