Detailed information of TA system TA177422

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 658915..659669
• Replicon: chromosome
• Accession: NZ_CP062997
• Organism: Klebsiella pneumoniae strain CriePir197

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A6L5EGB6
• Locus tag: GJJ12_RS03250
• Protein ID: WP_032669201.1
• Coordinates: 659292..659669 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: GJJ12_RS03245
• Protein ID: WP_074143921.1
• Coordinates: 658915..659241 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJJ12_RS03210	654946..655659	+	714	WP_032669194.1	WYL domain-containing protein	-
GJJ12_RS03215	655656..656291	+	636	WP_020837117.1	hypothetical protein	-
GJJ12_RS03220	656500..656731	+	232	Protein_632	DUF905 domain-containing protein	-
GJJ12_RS03225	656830..657651	+	822	WP_032669198.1	DUF945 domain-containing protein	-
GJJ12_RS03230	657682..658125	+	444	WP_032669199.1	antirestriction protein	-
GJJ12_RS03235	658137..658616	+	480	WP_032669200.1	DNA repair protein RadC	-
GJJ12_RS03240	658637..658858	+	222	WP_023567997.1	DUF987 domain-containing protein	-
GJJ12_RS03245	658915..659241	+	327	WP_074143921.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GJJ12_RS03250	659292..659669	+	378	WP_032669201.1	TA system toxin CbtA family protein	Toxin
GJJ12_RS03255	659666..660157	+	492	WP_032672023.1	hypothetical protein	-
GJJ12_RS03260	660187..660390	+	204	WP_032669203.1	DUF957 domain-containing protein	-
GJJ12_RS03265	660454..661302	+	849	WP_032669204.1	DUF4942 domain-containing protein	-
GJJ12_RS03275	661605..662111	+	507	WP_002917636.1	G/U mismatch-specific DNA glycosylase	-
GJJ12_RS03280	662211..664052	-	1842	WP_004174339.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	645359..659669	14310

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14157.93 Da
• Isoelectric Point: 6.2236

>T177422 WP_032669201.1 NZ_CP062997:659292-659669 [Klebsiella pneumoniae]
MQTQPLSSTQEATPRPSPVEIWQRLLSHLLDRHYGLTLNDTPFGNDGVIQEHIDAGISLCDAVNFIVEKYDLVRTDRRGF
NAETQSPLLSSIDILRARKATGLMTRNDYRTVTDITTGKYREVQP

Nucleotide

• Length: 378 bp

>T177422 NZ_CP083484:124877-125320 [Escherichia coli]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 11953.71 Da
• Isoelectric Point: 6.8397

>AT177422 WP_074143921.1 NZ_CP062997:658915-659241 [Klebsiella pneumoniae]
ITEPWWGLKRSITPCFGARLVQAGNRLHYLADRANITGQFSDADLRYLDQAFPLLLKQLELMLTSGELNPCHQHCVTLYA
KGLTCEADSLGSHGYVYLAIYPTPAATA

Nucleotide

• Length: 327 bp

>AT177422 NZ_CP083484:124650-124880 [Escherichia coli]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: A0A6L5EGB6