Detailed information of TA system TA177419

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 82540..83291
• Replicon: plasmid unnamed1
• Accession: NZ_CP062987
• Organism: Klebsiella pneumoniae strain CriePir26

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: H6U1U8
• Locus tag: GJJ26_RS26445
• Protein ID: WP_014386536.1
• Coordinates: 82540..83022 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: GJJ26_RS26450
• Protein ID: WP_004902250.1
• Coordinates: 83013..83291 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJJ26_RS26425 (GJJ26_026425)	78939..79586	-	648	WP_014386537.1	EcsC family protein	-
GJJ26_RS26430 (GJJ26_026430)	79613..80368	-	756	WP_004902235.1	DUF2971 domain-containing protein	-
GJJ26_RS26435 (GJJ26_026435)	80469..80861	-	393	WP_032442757.1	hypothetical protein	-
GJJ26_RS26440 (GJJ26_026440)	80966..81505	-	540	WP_004902239.1	hypothetical protein	-
GJJ26_RS26445 (GJJ26_026445)	82540..83022	-	483	WP_014386536.1	GNAT family N-acetyltransferase	Toxin
GJJ26_RS26450 (GJJ26_026450)	83013..83291	-	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
GJJ26_RS26455 (GJJ26_026455)	83410..83622	-	213	WP_004902255.1	hypothetical protein	-
GJJ26_RS26460 (GJJ26_026460)	83730..84071	-	342	WP_004902257.1	hypothetical protein	-
GJJ26_RS26465 (GJJ26_026465)	84900..85358	-	459	WP_014386535.1	hypothetical protein	-
GJJ26_RS26470 (GJJ26_026470)	86011..86466	-	456	WP_001166628.1	Hg(II)-responsive transcriptional regulator	-
GJJ26_RS26475 (GJJ26_026475)	86538..86903	+	366	WP_032430780.1	mercuric ion transporter MerT	-
GJJ26_RS26480 (GJJ26_026480)	86919..87200	+	282	WP_000732277.1	mercury resistance system periplasmic binding protein MerP	-
GJJ26_RS26485 (GJJ26_026485)	87228..87635	+	408	WP_000523860.1	organomercurial transporter MerC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	catA1 / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaCTX-M-15 / sul2 / aph(6)-Id / aph(3'')-Ib / sul1 / qacE / ant(3'')-Ia / dfrA15	-	1..146591	146591

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17662.45 Da
• Isoelectric Point: 8.9130

>T177419 WP_014386536.1 NZ_CP062987:c83022-82540 [Klebsiella pneumoniae]
MGMRAPESLTAEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T177419 NZ_CP083481:4319302-4319637 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAGCTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT177419 WP_004902250.1 NZ_CP062987:c83291-83013 [Klebsiella pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT177419 NZ_CP083481:4319054-4319302 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A2A5MBI1

Antitoxin

• AlphaFold DB: A0A071LPN3