Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5250509..5251134 Replicon chromosome
Accession NZ_CP062986
Organism Klebsiella pneumoniae strain CriePir26

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag GJJ26_RS25665 Protein ID WP_002882817.1
Coordinates 5250509..5250892 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag GJJ26_RS25670 Protein ID WP_004150355.1
Coordinates 5250892..5251134 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJJ26_RS25650 (GJJ26_025650) 5247875..5248777 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
GJJ26_RS25655 (GJJ26_025655) 5248774..5249409 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
GJJ26_RS25660 (GJJ26_025660) 5249406..5250335 + 930 WP_025368581.1 formate dehydrogenase accessory protein FdhE -
GJJ26_RS25665 (GJJ26_025665) 5250509..5250892 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
GJJ26_RS25670 (GJJ26_025670) 5250892..5251134 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
GJJ26_RS25675 (GJJ26_025675) 5251339..5252256 + 918 WP_025368582.1 alpha/beta hydrolase -
GJJ26_RS25680 (GJJ26_025680) 5252271..5253212 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
GJJ26_RS25685 (GJJ26_025685) 5253257..5253694 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
GJJ26_RS25690 (GJJ26_025690) 5253691..5254551 - 861 WP_004146232.1 virulence factor BrkB family protein -
GJJ26_RS25695 (GJJ26_025695) 5254545..5255144 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T177418 WP_002882817.1 NZ_CP062986:c5250892-5250509 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T177418 NZ_CP083481:4212688-4213095 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT177418 WP_004150355.1 NZ_CP062986:c5251134-5250892 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT177418 NZ_CP083481:4212441-4212707 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References