Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4655183..4655993 Replicon chromosome
Accession NZ_CP062986
Organism Klebsiella pneumoniae strain CriePir26

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag GJJ26_RS22890 Protein ID WP_004178461.1
Coordinates 4655183..4655716 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag GJJ26_RS22895 Protein ID WP_002887278.1
Coordinates 4655727..4655993 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJJ26_RS22885 (GJJ26_022890) 4654014..4655135 + 1122 WP_002887282.1 cupin domain-containing protein -
GJJ26_RS22890 (GJJ26_022895) 4655183..4655716 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
GJJ26_RS22895 (GJJ26_022900) 4655727..4655993 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
GJJ26_RS22900 (GJJ26_022905) 4656096..4657529 - 1434 WP_021463709.1 Cu(+)/Ag(+) sensor histidine kinase -
GJJ26_RS22905 (GJJ26_022910) 4657519..4658202 - 684 WP_020804437.1 copper response regulator transcription factor CusR -
GJJ26_RS22910 (GJJ26_022915) 4658374..4659759 + 1386 WP_025368486.1 efflux transporter outer membrane subunit -
GJJ26_RS22915 (GJJ26_022920) 4659777..4660109 + 333 WP_025368487.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T177415 WP_004178461.1 NZ_CP062986:c4655716-4655183 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T177415 NZ_CP083481:3536751-3536858 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CATGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT177415 WP_002887278.1 NZ_CP062986:c4655993-4655727 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT177415 NZ_CP083481:c3536702-3536639 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References