Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 3174051..3174682 Replicon chromosome
Accession NZ_CP062986
Organism Klebsiella pneumoniae strain CriePir26

Toxin (Protein)


Gene name hicA Uniprot ID A0A1Q8YTV3
Locus tag GJJ26_RS15730 Protein ID WP_012542177.1
Coordinates 3174506..3174682 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A3Q9U6R4
Locus tag GJJ26_RS15725 Protein ID WP_017898984.1
Coordinates 3174051..3174458 (-) Length 136 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJJ26_RS15690 (GJJ26_015690) 3169415..3169849 - 435 WP_012542168.1 phage terminase small subunit P27 family -
GJJ26_RS15695 (GJJ26_015695) 3170097..3170528 - 432 WP_023279521.1 hypothetical protein -
GJJ26_RS15700 (GJJ26_015700) 3170525..3170842 - 318 WP_023279522.1 hypothetical protein -
GJJ26_RS15705 (GJJ26_015705) 3170794..3171156 - 363 WP_014228901.1 HNH endonuclease -
GJJ26_RS15710 (GJJ26_015710) 3172281..3172631 - 351 WP_017898986.1 hypothetical protein -
GJJ26_RS15715 (GJJ26_015715) 3172628..3173125 - 498 WP_023279523.1 lysozyme -
GJJ26_RS15720 (GJJ26_015720) 3173125..3173340 - 216 WP_017880269.1 class II holin family protein -
GJJ26_RS15725 (GJJ26_015725) 3174051..3174458 - 408 WP_017898984.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GJJ26_RS15730 (GJJ26_015730) 3174506..3174682 - 177 WP_012542177.1 type II toxin-antitoxin system HicA family toxin Toxin
GJJ26_RS15735 (GJJ26_015735) 3175046..3176830 + 1785 WP_032415155.1 ATP-binding protein -
GJJ26_RS15740 (GJJ26_015740) 3176850..3177884 + 1035 WP_071600377.1 DNA cytosine methyltransferase -
GJJ26_RS15745 (GJJ26_015745) 3177909..3178250 - 342 WP_017898982.1 antiterminator Q family protein -
GJJ26_RS15750 (GJJ26_015750) 3178263..3179294 - 1032 WP_071058942.1 DUF968 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3140651..3191801 51150


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-61)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6632.83 Da        Isoelectric Point: 11.0174

>T177413 WP_012542177.1 NZ_CP062986:c3174682-3174506 [Klebsiella pneumoniae]
VKQSELRRWLAAQGAEFKDGTNHLKIYLNGKQTVMPRHPGKEIPEPLRKAILKQLGIK

Download         Length: 177 bp

>T177413 NZ_CP083481:3535784-3535891 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGCATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 136 a.a.        Molecular weight: 14959.96 Da        Isoelectric Point: 4.4277

>AT177413 WP_017898984.1 NZ_CP062986:c3174458-3174051 [Klebsiella pneumoniae]
MRYPVIFEHDETGWAVFFPDIPEAMTGGETREEALEMAQDALVTAFDFYFDDRREIPAPSAEGDAFVEVPASVAAKVLLL
NRLVSTNTSNADLARLINTRPQEVQRIVSLGHSTKIDTIQKALSALGQKMEIVVH

Download         Length: 408 bp

>AT177413 NZ_CP083481:c3535726-3535672 [Escherichia coli]
CAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q8YTV3

Antitoxin

Source ID Structure
AlphaFold DB A0A3Q9U6R4

References