Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2659629..2661217 Replicon chromosome
Accession NZ_CP062986
Organism Klebsiella pneumoniae strain CriePir26

Toxin (Protein)


Gene name hipA Uniprot ID W8UI36
Locus tag GJJ26_RS13185 Protein ID WP_002904792.1
Coordinates 2659629..2660951 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A0H3GXD8
Locus tag GJJ26_RS13190 Protein ID WP_002904790.1
Coordinates 2660951..2661217 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJJ26_RS13170 (GJJ26_013175) 2654685..2657186 + 2502 WP_004151634.1 fimbria/pilus outer membrane usher protein -
GJJ26_RS13175 (GJJ26_013180) 2657174..2658178 + 1005 WP_002904798.1 fimbrial protein -
GJJ26_RS13180 (GJJ26_013185) 2658320..2659612 + 1293 WP_002904794.1 glycoside hydrolase family 10 protein -
GJJ26_RS13185 (GJJ26_013190) 2659629..2660951 - 1323 WP_002904792.1 type II toxin-antitoxin system HipA family toxin Toxin
GJJ26_RS13190 (GJJ26_013195) 2660951..2661217 - 267 WP_002904790.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
GJJ26_RS13195 (GJJ26_013200) 2661483..2661809 - 327 WP_002904788.1 hydroxyisourate hydrolase -
GJJ26_RS13200 (GJJ26_013205) 2661806..2662306 - 501 WP_002904787.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
GJJ26_RS13205 (GJJ26_013210) 2662284..2663663 - 1380 WP_002904785.1 nucleobase:cation symporter-2 family protein -
GJJ26_RS13210 (GJJ26_013215) 2664009..2665163 + 1155 WP_002904783.1 FAD-dependent urate hydroxylase HpxO -
GJJ26_RS13215 (GJJ26_013220) 2665144..2666067 - 924 WP_002904781.1 LysR family hpxDE operon transcriptional regulator HpxR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - mgtC / iroE 2459069..2696701 237632


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(149-402)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49171.46 Da        Isoelectric Point: 7.5357

>T177412 WP_002904792.1 NZ_CP062986:c2660951-2659629 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

>T177412 NZ_CP083481:c3112605-3112294 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9906.27 Da        Isoelectric Point: 8.5912

>AT177412 WP_002904790.1 NZ_CP062986:c2661217-2660951 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Download         Length: 267 bp

>AT177412 NZ_CP083481:c3112293-3112003 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E1CEC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GXD8

References