Detailed information of TA system TA177400

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4894886..4895721
• Replicon: chromosome
• Accession: NZ_CP062985
• Organism: Escherichia coli UTI89

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q1R2L4
• Locus tag: IIE08_RS23470
• Protein ID: WP_001094426.1
• Coordinates: 4895344..4895721 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: Q1R2L5
• Locus tag: IIE08_RS23465
• Protein ID: WP_001285575.1
• Coordinates: 4894886..4895254 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IIE08_RS23430	4890779..4891459	+	681	WP_001282919.1	WYL domain-containing protein	-
IIE08_RS23435	4891607..4892284	+	678	WP_001097301.1	hypothetical protein	-
IIE08_RS23440	4892290..4892523	+	234	WP_001278290.1	DUF905 family protein	-
IIE08_RS23445	4892613..4893431	+	819	WP_001175160.1	DUF945 domain-containing protein	-
IIE08_RS23450	4893522..4894007	+	486	WP_000213697.1	antirestriction protein	-
IIE08_RS23455	4894022..4894498	+	477	WP_001298074.1	RadC family protein	-
IIE08_RS23460	4894585..4894806	+	222	WP_000692350.1	DUF987 domain-containing protein	-
IIE08_RS23465	4894886..4895254	+	369	WP_001285575.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
IIE08_RS23470	4895344..4895721	+	378	WP_001094426.1	TA system toxin CbtA family protein	Toxin
IIE08_RS23475	4895718..4896206	+	489	WP_000761683.1	hypothetical protein	-
IIE08_RS23480	4896218..4896415	+	198	WP_000839272.1	DUF957 domain-containing protein	-
IIE08_RS23485	4896494..4896652	+	159	WP_001467148.1	hypothetical protein	-
IIE08_RS23490	4897664..4899640	+	1977	WP_000464678.1	hypothetical protein	-
IIE08_RS23495	4900039..4900218	-	180	WP_001298063.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB / fimE / fimA / fimI / fimC	4882183..4909777	27594

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14072.99 Da
• Isoelectric Point: 7.3523

>T177400 WP_001094426.1 NZ_CP062985:4895344-4895721 [Escherichia coli UTI89]
MNTLPDTHIREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T177400 NZ_CP083481:c752476-752300 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCGTGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13709.52 Da
• Isoelectric Point: 6.6258

>AT177400 WP_001285575.1 NZ_CP062985:4894886-4895254 [Escherichia coli UTI89]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT177400 NZ_CP083481:c752254-751838 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A454ABD6

Antitoxin

• AlphaFold DB: A0A454ABF5