Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3219082..3219736 Replicon chromosome
Accession NZ_CP062985
Organism Escherichia coli UTI89

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag IIE08_RS15545 Protein ID WP_000244781.1
Coordinates 3219082..3219489 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag IIE08_RS15550 Protein ID WP_000354046.1
Coordinates 3219470..3219736 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IIE08_RS15525 3215039..3216772 - 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -
IIE08_RS15530 3216778..3217488 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IIE08_RS15535 3217513..3218409 - 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
IIE08_RS15540 3218521..3219042 + 522 WP_001055874.1 flavodoxin FldB -
IIE08_RS15545 3219082..3219489 - 408 WP_000244781.1 protein YgfX Toxin
IIE08_RS15550 3219470..3219736 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
IIE08_RS15555 3219979..3220959 + 981 WP_000886078.1 tRNA-modifying protein YgfZ -
IIE08_RS15560 3221036..3221695 - 660 WP_000250274.1 hemolysin III family protein -
IIE08_RS15565 3221859..3222170 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
IIE08_RS15570 3222215..3223648 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T177397 WP_000244781.1 NZ_CP062985:c3219489-3219082 [Escherichia coli UTI89]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T177397 NZ_CP083481:284664-284767 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT177397 WP_000354046.1 NZ_CP062985:c3219736-3219470 [Escherichia coli UTI89]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT177397 NZ_CP083481:c284805-284534 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGCCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References