Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2216967..2217798 Replicon chromosome
Accession NZ_CP062985
Organism Escherichia coli UTI89

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag IIE08_RS10790 Protein ID WP_000854814.1
Coordinates 2217424..2217798 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1PWQ3
Locus tag IIE08_RS10785 Protein ID WP_001285586.1
Coordinates 2216967..2217335 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IIE08_RS10750 2212025..2213095 + 1071 WP_000102631.1 patatin-like phospholipase family protein -
IIE08_RS10755 2213231..2213908 + 678 WP_001362823.1 hypothetical protein -
IIE08_RS10760 2213924..2214334 + 411 WP_000846704.1 hypothetical protein -
IIE08_RS10765 2214555..2215376 + 822 WP_001234570.1 DUF945 domain-containing protein -
IIE08_RS10770 2215639..2216112 + 474 WP_000855071.1 antirestriction protein -
IIE08_RS10775 2216127..2216603 + 477 WP_001298074.1 RadC family protein -
IIE08_RS10780 2216672..2216893 + 222 WP_000692323.1 DUF987 domain-containing protein -
IIE08_RS10785 2216967..2217335 + 369 WP_001285586.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
IIE08_RS10790 2217424..2217798 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
IIE08_RS10795 2217795..2217989 + 195 WP_000988601.1 hypothetical protein -
IIE08_RS10800 2218002..2218115 + 114 WP_001161660.1 DUF957 domain-containing protein -
IIE08_RS10805 2218404..2218550 - 147 Protein_2114 transposase domain-containing protein -
IIE08_RS10810 2218604..2218786 + 183 WP_001016348.1 hypothetical protein -
IIE08_RS10815 2218887..2219216 - 330 WP_000450409.1 DUF496 family protein -
IIE08_RS10820 2219388..2220446 - 1059 WP_001200890.1 FUSC family protein -
IIE08_RS10825 2220644..2221117 - 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
IIE08_RS10830 2221236..2222401 - 1166 Protein_2119 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T177394 WP_000854814.1 NZ_CP062985:2217424-2217798 [Escherichia coli UTI89]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T177394 NZ_CP083479:145677-145826 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13563.41 Da        Isoelectric Point: 5.0468

>AT177394 WP_001285586.1 NZ_CP062985:2216967-2217335 [Escherichia coli UTI89]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT177394 NZ_CP083479:c145633-145572 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB S1PWQ3

References