Detailed information of TA system TA177375

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 53024..53382
• Replicon: chromosome
• Accession: NZ_CP062985
• Organism: Escherichia coli UTI89

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: IIE08_RS00250
• Protein ID: WP_001332330.1
• Coordinates: 53260..53382 (+)
• Length: 41 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: IIE08_RS00245
• Protein ID: WP_000125566.1
• Coordinates: 53024..53257 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IIE08_RS00220	48097..48384	+	288	WP_000203741.1	ferredoxin-like protein FixX	-
IIE08_RS00225	48443..49774	+	1332	WP_001183207.1	MFS transporter	-
IIE08_RS00230	49882..50412	+	531	WP_000600749.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
IIE08_RS00235	50405..52267	+	1863	WP_000377122.1	glutathione-regulated potassium-efflux system protein KefC	-
IIE08_RS00240	52459..52938	+	480	WP_000624375.1	type 3 dihydrofolate reductase	-
IIE08_RS00245	53024..53257	+	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
IIE08_RS00250	53260..53382	+	123	WP_001332330.1	CcdB family protein	Toxin
IIE08_RS00255	53423..53575	+	153	WP_146692624.1	CcdB family protein	-
IIE08_RS00260	53572..54420	-	849	WP_000257201.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
IIE08_RS00265	54427..54804	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
IIE08_RS00270	54807..55628	-	822	WP_001065363.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
IIE08_RS00275	55625..56614	-	990	WP_000241229.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
IIE08_RS00280	56614..57900	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-39)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4563.34 Da
• Isoelectric Point: 10.9266

>T177375 WP_001332330.1 NZ_CP062985:53260-53382 [Escherichia coli UTI89]
MQFTVYRSRGRNAAFPFVIDVTSDIVGEINRRIVIPLTPI

Nucleotide

• Length: 123 bp

>T177375 NZ_CP083478:1325861-1326388 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT177375 WP_000125566.1 NZ_CP062985:53024-53257 [Escherichia coli UTI89]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT177375 NZ_CP083478:1325588-1325854 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9