Detailed information of TA system TA177372

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1214760..1215676
• Replicon: chromosome
• Accession: NZ_CP062984
• Organism: Bacillus amyloliquefaciens strain PP19 isolate PP19

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: IL989_RS06245
• Protein ID: WP_193351641.1
• Coordinates: 1214930..1215676 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: IL989_RS06240
• Protein ID: WP_003154807.1
• Coordinates: 1214760..1214930 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IL989_RS06210	1211626..1212024	+	399	WP_013351961.1	hypothetical protein	-
IL989_RS06215	1212011..1212208	+	198	WP_013351962.1	XkdX family protein	-
IL989_RS06220	1212257..1213018	+	762	WP_013351963.1	hypothetical protein	-
IL989_RS06225	1213072..1213335	+	264	WP_013351964.1	hemolysin XhlA family protein	-
IL989_RS06230	1213349..1213612	+	264	WP_013351965.1	phage holin	-
IL989_RS06235	1213626..1214504	+	879	WP_014470491.1	N-acetylmuramoyl-L-alanine amidase	-
IL989_RS06240	1214760..1214930	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
IL989_RS06245	1214930..1215676	-	747	WP_193351641.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
IL989_RS06250	1215784..1216782	-	999	WP_013351968.1	inorganic phosphate transporter	-
IL989_RS06255	1216795..1217412	-	618	WP_003154804.1	DUF47 domain-containing protein	-
IL989_RS06260	1217697..1219013	-	1317	WP_193351642.1	amino acid permease	-
IL989_RS06265	1219337..1220287	+	951	WP_193351643.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29082.59 Da
• Isoelectric Point: 4.6839

>T177372 WP_193351641.1 NZ_CP062984:c1215676-1214930 [Bacillus amyloliquefaciens]
MLLFYQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAVDVQSRLNRKDVYYDQKGRLVLIPFTVQNRQYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T177372 NZ_CP083478:c541346-541173 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT177372 WP_003154807.1 NZ_CP062984:c1214930-1214760 [Bacillus amyloliquefaciens]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT177372 NZ_CP083478:c541670-541374 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: I2HQ14