Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 468594..469231 Replicon chromosome
Accession NZ_CP062984
Organism Bacillus amyloliquefaciens strain PP19 isolate PP19

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag IL989_RS02375 Protein ID WP_003156187.1
Coordinates 468881..469231 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag IL989_RS02370 Protein ID WP_003156188.1
Coordinates 468594..468875 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IL989_RS02350 464960..465559 - 600 WP_013351092.1 rhomboid family intramembrane serine protease -
IL989_RS02355 465652..466017 + 366 WP_013351093.1 holo-ACP synthase -
IL989_RS02360 466182..467189 + 1008 WP_013351094.1 outer membrane lipoprotein carrier protein LolA -
IL989_RS02365 467306..468475 + 1170 WP_013351095.1 alanine racemase -
IL989_RS02370 468594..468875 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
IL989_RS02375 468881..469231 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
IL989_RS02380 469352..470173 + 822 WP_193351327.1 STAS domain-containing protein -
IL989_RS02385 470178..470543 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
IL989_RS02390 470546..470947 + 402 WP_013351097.1 anti-sigma regulatory factor -
IL989_RS02395 470959..471966 + 1008 WP_193351328.1 PP2C family protein-serine/threonine phosphatase -
IL989_RS02400 472030..472359 + 330 WP_014469786.1 anti-sigma factor antagonist -
IL989_RS02405 472356..472838 + 483 WP_003156173.1 anti-sigma B factor RsbW -
IL989_RS02410 472804..473592 + 789 WP_013351101.1 RNA polymerase sigma factor SigB -
IL989_RS02415 473592..474194 + 603 WP_045508359.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T177371 WP_003156187.1 NZ_CP062984:468881-469231 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T177371 NZ_CP083478:c439016-438666 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT177371 WP_003156188.1 NZ_CP062984:468594-468875 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT177371 NZ_CP083478:c439261-439010 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References