Detailed information of TA system TA177370

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 5265596..5266616
• Replicon: chromosome
• Accession: NZ_CP062983
• Organism: Anaerolinea sp. 'rifampicinis'

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: G4Y79_RS22665
• Protein ID: WP_195170524.1
• Coordinates: 5265596..5266165 (-)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: G4Y79_RS22670
• Protein ID: WP_195170525.1
• Coordinates: 5266167..5266616 (-)
• Length: 150 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4Y79_RS22650	5261550..5263040	+	1491	WP_195170521.1	DUF4139 domain-containing protein	-
G4Y79_RS22655	5263156..5264808	-	1653	WP_195170522.1	chaperonin GroEL	-
G4Y79_RS22660	5264944..5265240	-	297	WP_195170523.1	co-chaperone GroES	-
G4Y79_RS22665	5265596..5266165	-	570	WP_195170524.1	PIN domain-containing protein	Toxin
G4Y79_RS22670	5266167..5266616	-	450	WP_195170525.1	helix-turn-helix domain-containing protein	Antitoxin
G4Y79_RS22675	5266800..5268368	-	1569	WP_195170526.1	serine/threonine protein kinase	-
G4Y79_RS22680	5268506..5268793	-	288	WP_195173353.1	hypothetical protein	-
G4Y79_RS22685	5269166..5270647	+	1482	WP_195170527.1	RHS repeat-associated core domain-containing protein	-
G4Y79_RS22690	5270788..5271093	+	306	WP_195170528.1	DUF1308 domain-containing protein	-
G4Y79_RS22695	5271097..5271417	+	321	WP_195170529.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Helix-turn-helix domain, group 17 (77-121)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21698.04 Da
• Isoelectric Point: 6.6424

>T177370 WP_195170524.1 NZ_CP062983:c5266165-5265596 [Anaerolinea sp. 'rifampicinis']
MSYYTALLDANILYPAPIRDMMVQLAVEDLFRAKWTEDIHREWIEALLRNEPYRERAALERTRNLMDKATRDSLVTGYKP
LINSLTLPDPDDRHVLAAAIVGRCDVIVTQNLHDFPESVVKPYGIEIQHPDDFLANHLNLAPGKFCVAVRKVRARLKNPR
FTVQEYLATLTRQGLVVTVAELEQYAQLI

Nucleotide

• Length: 570 bp

>T177370 NZ_CP083475:c32706-31843 [Enterococcus faecium]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGGTATAATTTGGTGATCGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTATATAACAGAGAAGGAGTAAAACTCTATTCAA
GTTTGGAAACACCTTCTATTAGTCCAAAAGAAACCTTAGAAAAAGAATTGAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTAGAACGAATAGAGCAAAAAATGGTTCTAAATAAACACCAAGAGACACCTGAATTTAAAGCAATTCAACAAAA
ATTGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin

Protein

• Length: 150 a.a.
• Molecular weight: 16743.05 Da
• Isoelectric Point: 4.7536

>AT177370 WP_195170525.1 NZ_CP062983:c5266616-5266167 [Anaerolinea sp. 'rifampicinis']
MLAYRHRPPNEEESSIARNSKQLLAQYARENESLTVHVTDQEGTIELPAGAVSLLMDILGAMASGQGITLIPENAELTTV
QAAEVLNVSRPFLIKLLEEGKIPYRKVGKHRRILMEDVMNYKHAIDEERESILDQLVADAQEQNMGYEK

Nucleotide

• Length: 450 bp

>AT177370 NZ_CP083475:c32980-32708 [Enterococcus faecium]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA