Detailed information of TA system TA177367

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 251493..252123
• Replicon: chromosome
• Accession: NZ_CP062974
• Organism: Pontibacillus sp. ALD_SL1

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: A0A0A2UP65
• Locus tag: IMZ31_RS01410
• Protein ID: WP_036786621.1
• Coordinates: 251773..252123 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: IMZ31_RS01405
• Protein ID: WP_188655955.1
• Coordinates: 251493..251768 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMZ31_RS01385	246978..247334	+	357	WP_207530264.1	holo-ACP synthase	-
IMZ31_RS01390	247410..248933	+	1524	WP_207528910.1	NAD(P)H-hydrate dehydratase	-
IMZ31_RS01395	248984..250009	+	1026	WP_207528911.1	outer membrane lipoprotein carrier protein LolA	-
IMZ31_RS01400	250161..251342	+	1182	WP_207528912.1	alanine racemase	-
IMZ31_RS01405	251493..251768	+	276	WP_188655955.1	antitoxin	Antitoxin
IMZ31_RS01410	251773..252123	+	351	WP_036786621.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
IMZ31_RS01415	252355..253224	+	870	WP_036786619.1	RsbT co-antagonist protein RsbRA	-
IMZ31_RS01420	253221..253586	+	366	WP_198135876.1	STAS domain-containing protein	-
IMZ31_RS01425	253591..253992	+	402	WP_036786615.1	anti-sigma regulatory factor	-
IMZ31_RS01430	254007..255017	+	1011	WP_207528913.1	SpoIIE family protein phosphatase	-
IMZ31_RS01435	255085..255420	+	336	WP_036786611.1	STAS domain-containing protein	-
IMZ31_RS01440	255420..255899	+	480	WP_036786609.1	anti-sigma B factor RsbW	-
IMZ31_RS01445	255871..256662	+	792	WP_188655951.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12962.97 Da
• Isoelectric Point: 4.8668

>T177367 WP_036786621.1 NZ_CP062974:251773-252123 [Pontibacillus sp. ALD_SL1]
MIVKRGDVYFADLSPVVGSEQGGVRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAERYGFERDSVILLEQI
RTIDKQRLTDKITHLDEAMMSRVDEALQISLGLIDF

Nucleotide

• Length: 351 bp

>T177367 NZ_CP083474:62809-63081 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACATATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10549.00 Da
• Isoelectric Point: 5.1990

>AT177367 WP_188655955.1 NZ_CP062974:251493-251768 [Pontibacillus sp. ALD_SL1]
VSDSSQEIVVKLPQHLLKELDGMVQLENGDRSDFIYQATKMYLRERKKRHIRESMRQGYMEMAKINLNIASEAFQAEEEA
ETTCERLVSGV

Nucleotide

• Length: 276 bp

>AT177367 NZ_CP083474:62546-62809 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTTGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Structures

Toxin

• AlphaFold DB: A0A0A2UP65