Detailed information of TA system TA177361

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/RelB(antitoxin)
• Location: 1869235..1869871
• Replicon: chromosome
• Accession: NZ_CP062951
• Organism: Bifidobacterium longum subsp. infantis strain JCM 11347

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: BL1347_RS08370
• Protein ID: WP_193641407.1
• Coordinates: 1869515..1869871 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: D6PAX1
• Locus tag: BL1347_RS08365
• Protein ID: WP_012577921.1
• Coordinates: 1869235..1869528 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BL1347_RS08340	1864384..1867062	-	2679	WP_193641406.1	alanine--tRNA ligase	-
BL1347_RS08345	1867191..1867427	-	237	WP_007053386.1	hypothetical protein	-
BL1347_RS08350	1867427..1867825	-	399	WP_007052133.1	DUF948 domain-containing protein	-
BL1347_RS08355	1867970..1868644	-	675	WP_193642262.1	histidine phosphatase family protein	-
BL1347_RS08360	1868876..1869163	+	288	Protein_1620	IS30 family transposase	-
BL1347_RS08365	1869235..1869528	+	294	WP_012577921.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BL1347_RS08370	1869515..1869871	+	357	WP_193641407.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BL1347_RS08375	1870048..1870650	-	603	WP_193641408.1	transglutaminase family protein	-
BL1347_RS08380	1870755..1871111	-	357	WP_193641409.1	SdpI family protein	-
BL1347_RS08385	1871202..1871687	+	486	WP_193641410.1	cytidine deaminase	-
BL1347_RS08390	1871825..1872607	+	783	WP_193641411.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
BL1347_RS08395	1872787..1873413	-	627	WP_007052130.1	30S ribosomal protein S4	-
BL1347_RS08400	1873612..1874604	-	993	WP_193641412.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-111)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (11-53)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13344.33 Da
• Isoelectric Point: 8.9603

>T177361 WP_193641407.1 NZ_CP062951:1869515-1869871 [Bifidobacterium longum subsp. infantis]
MMKTDPRQFEIWWVPFAFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSNLFLDDGPTGRLSAYDAVRVAWAINELYPGLLA

Nucleotide

• Length: 357 bp

>T177361 NZ_CP083454:372626-372928 [Stenotrophomonas maltophilia]
ATGGCTATCAAGAAGAAAGTTGAACTCGAAGCATTTGATGTGGCCGAACACCTTCATACACCGAAGGAAATGGCGGCCTA
TCTCGATGCATGCATCGAAGAAAGCGACGGCGATTCGGCATTCATCGCCAAGGCGCTGGGCGATATCGCGCGTGCCCAAG
GGATGAGCAAGGTCGCACGTGCGGCGGGCCTGTCGCGGGAGAGTCTGTATCGTGCCCTGTCTGGCGAGCGCAGCCCTGAT
TTCTCGACCATCCTGAAGGTGACTCGTGCCCTGGGTGTGCGCCTGCATGCCAGCGCTGCATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10866.31 Da
• Isoelectric Point: 4.2896

>AT177361 WP_012577921.1 NZ_CP062951:1869235-1869528 [Bifidobacterium longum subsp. infantis]
MAIKEKTTISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTPQ
IRDGAVILPEGWDDDED

Nucleotide

• Length: 294 bp

>AT177361 NZ_CP083454:372280-372585 [Stenotrophomonas maltophilia]
ATGAAGATCCAGCGTACCCGTCAGTTCGCGACATGGATTGACGCTCTCAAGGACGTAACCGCACGCGCTCGCATCCTGGC
CCGTATCGGTCGGCTCGCTGAAGGCCATCCTGGTGATCACCGCTATCTGGCGGATGGCGTTTCTGAATTGCGTATCGATG
CAGGACCCGGTTACCGCGTGTACTACACCCAGCGGGGCAGGCAGTTGGTGATTCTTCTGGTCGGAGGCGACAAGGGTTCG
CAACAGCGCGATATAGAGAAGGCCAAGGAGATTGCGCGTGCCCTGCGAGCATCGAACTGGTTGTAG

Structures

Antitoxin

• AlphaFold DB: A0A1S2VTZ6