Detailed information of TA system TA177354

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 1461069..1461677
• Replicon: chromosome
• Accession: NZ_CP062949
• Organism: Bifidobacterium longum subsp. longum strain JCM 7050

Toxin (Protein)

• Gene name: relE
• Uniprot ID: I3BGL7
• Locus tag: BL7050_RS06045
• Protein ID: WP_007056255.1
• Coordinates: 1461069..1461404 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: I3BGP0
• Locus tag: BL7050_RS06050
• Protein ID: WP_007056990.1
• Coordinates: 1461408..1461677 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BL7050_RS06010	1456165..1456839	+	675	WP_077322431.1	YidC/Oxa1 family membrane protein insertase	-
BL7050_RS06015	1456941..1457561	+	621	WP_007054239.1	NUDIX hydrolase	-
BL7050_RS06020	1457590..1457913	+	324	WP_012471871.1	amidohydrolase	-
BL7050_RS06025	1458950..1459591	-	642	WP_131268534.1	moaA protein	-
BL7050_RS06030	1459805..1459984	+	180	WP_007056286.1	antitoxin VbhA family protein	-
BL7050_RS06035	1459981..1460565	+	585	WP_193020894.1	Fic family protein	-
BL7050_RS06040	1460768..1461052	-	285	WP_007056282.1	hypothetical protein	-
BL7050_RS06045	1461069..1461404	-	336	WP_007056255.1	type II toxin-antitoxin system YafQ family toxin	Toxin
BL7050_RS06050	1461408..1461677	-	270	WP_007056990.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BL7050_RS06055	1461862..1463031	-	1170	WP_007056274.1	ABC transporter permease	-
BL7050_RS06060	1463028..1463708	-	681	WP_003829413.1	ABC transporter ATP-binding protein	-
BL7050_RS06065	1463705..1464595	-	891	WP_007056280.1	hypothetical protein	-
BL7050_RS06070	1464592..1465107	-	516	WP_032685213.1	hypothetical protein	-
BL7050_RS06075	1465233..1465928	+	696	WP_003829410.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1442273..1484696	42423

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-96)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (3-87)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12650.60 Da
• Isoelectric Point: 9.1088

>T177354 WP_007056255.1 NZ_CP062949:c1461404-1461069 [Bifidobacterium longum subsp. longum]
MLKPDYTAAFQRDIKKLKRKHTDLRPLKEVIRLVLEDTADSKEVLRRRHRAHTLTGDLNGVLECHIGNAGDWLLLWIRDD
GTAMFMRTGSHDELFGQIAGLHPALNQPTAI

Nucleotide

• Length: 336 bp

>T177354 NZ_CP083449:65512-65763 [Pantoea eucrina]
ATGAAAGCAATAAGCTACAGTGAAGCAAGACAGAATCTGTCTGCAACAATGCTGCAAACGGTTGAGGACAGAGCCCCGGT
GCTTATTACCCGTCAGAACGGTGAATCATGCGTATTGATGTCACTTGAGGAGTACAACTCACTTGAAGAAACTGCATACC
TGCTTCGGTCTCCGAAAAATGCGCGCAGACTTATGGACTCCATTGAAAGCCTTAAAAGCGGTAACGGTATGGAGAGGGAC
GTCATTGAGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9844.16 Da
• Isoelectric Point: 4.5742

>AT177354 WP_007056990.1 NZ_CP062949:c1461677-1461408 [Bifidobacterium longum subsp. longum]
VSMASVTVRVDADTKQKAANIAEDLGLDLSSVTRAFYKQIVREKRIPLNLSYTIVPPETMEALDEAKNRLADGQPRFNSA
DEMFDSLGI

Nucleotide

• Length: 270 bp

>AT177354 NZ_CP083449:65760-66014 [Pantoea eucrina]
GTGAAACTGACATGGTCTGCTGAAGCATGGGAAGATTATCTTTACTGGCAGGATACGGATAAAAAAACGGTAAAAAAAAT
AAATGAGCTCATTAAGGATGCCAGCAGGACGCCATTTGAGGGGAAGGGAAAGCCCGAACCTCTAAAGCATCATCTTGCGG
GGTTCTGGTCCCGGAGGATCACAGCTGAACATCGGCTTGTCTACGCCGTTAGCGATGATGCTCTGCTGATAGCATCCTGC
CGCTATCACTACTAA

Structures

Toxin

• AlphaFold DB: I3BGL7

Antitoxin

• AlphaFold DB: I3BGP0