Detailed information of TA system TA177333

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 35167..35775
• Replicon: chromosome
• Accession: NZ_CP062940
• Organism: Bifidobacterium longum subsp. longum strain JCM 11343

Toxin (Protein)

• Gene name: relE
• Uniprot ID: I3BGL7
• Locus tag: BL1343_RS00190
• Protein ID: WP_007056255.1
• Coordinates: 35167..35502 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: I3BGP0
• Locus tag: BL1343_RS00195
• Protein ID: WP_007056990.1
• Coordinates: 35506..35775 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BL1343_RS00145	30264..30938	+	675	WP_077322431.1	YidC/Oxa1 family membrane protein insertase	-
BL1343_RS00150	31040..31660	+	621	WP_131269761.1	NUDIX hydrolase	-
BL1343_RS00155	31689..32012	+	324	WP_074709766.1	amidohydrolase	-
BL1343_RS00160	32176..32322	-	147	WP_007056267.1	hypothetical protein	-
BL1343_RS00165	32662..32955	-	294	WP_012471870.1	hypothetical protein	-
BL1343_RS00170	33048..33689	-	642	WP_007056262.1	hypothetical protein	-
BL1343_RS00175	33903..34082	+	180	WP_007056286.1	antitoxin VbhA family protein	-
BL1343_RS00180	34079..34663	+	585	WP_007056278.1	Fic family protein	-
BL1343_RS00185	34866..35150	-	285	WP_007056282.1	hypothetical protein	-
BL1343_RS00190	35167..35502	-	336	WP_007056255.1	type II toxin-antitoxin system YafQ family toxin	Toxin
BL1343_RS00195	35506..35775	-	270	WP_007056990.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BL1343_RS00200	35960..37129	-	1170	WP_007056274.1	ABC transporter permease	-
BL1343_RS00205	37126..37806	-	681	WP_003829413.1	ABC transporter ATP-binding protein	-
BL1343_RS00210	37803..38693	-	891	WP_007056280.1	hypothetical protein	-
BL1343_RS00215	38690..39205	-	516	WP_032685213.1	hypothetical protein	-
BL1343_RS00220	39331..40026	+	696	WP_003829410.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-96)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (3-87)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12650.60 Da
• Isoelectric Point: 9.1088

>T177333 WP_007056255.1 NZ_CP062940:c35502-35167 [Bifidobacterium longum subsp. longum]
MLKPDYTAAFQRDIKKLKRKHTDLRPLKEVIRLVLEDTADSKEVLRRRHRAHTLTGDLNGVLECHIGNAGDWLLLWIRDD
GTAMFMRTGSHDELFGQIAGLHPALNQPTAI

Nucleotide

• Length: 336 bp

>T177333 NZ_CP083444:615236-615544 [Bartonella taylorii]
ATGATAACCATTCATAAAACTACAGAATTTGATACTTGGCTTAAAAAACTCAACGATAAAGGCGTTAAAGCTATTATTCT
TCAACGTGTTATACGATTAAGACAAGGTCTTTTAGGTGATGTTAAATTCTTTAATGGCATTGGTGAATTACGTATCCACT
ATGGGGCTGGTTACAGAATTTATTTTACCCAAAAAGGCTCTGATTTTATCCTTTTGTTATGTGGTGGAGATAAATCAACG
CAGAAAAGAGATATTGAACGAGCGTTAAAGTTAAAAGAGGAGTACAGTGATGAAAACTACACCATTTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9844.16 Da
• Isoelectric Point: 4.5742

>AT177333 WP_007056990.1 NZ_CP062940:c35775-35506 [Bifidobacterium longum subsp. longum]
VSMASVTVRVDADTKQKAANIAEDLGLDLSSVTRAFYKQIVREKRIPLNLSYTIVPPETMEALDEAKNRLADGQPRFNSA
DEMFDSLGI

Nucleotide

• Length: 270 bp

>AT177333 NZ_CP083444:615525-615809 [Bartonella taylorii]
ATGAAAACTACACCATTTAAACCAGAAGAATATCTTGAAACCACTGAGGAACAACAAATATTTCTCAATGAAGCCTTCAA
AACAGGTGATGCTGCTCACATAGCGGATGCCCTTGGTATTGTTGCACGTGCTCAAAATATGAGAGCCTTAGCAAAAGAAA
CCAATCGTGAACGCAGTGGTTTATATCGCTCCTTAAGCAAAACAGGTGACCCTAAACTTTCTACCTTAGTCGCTGTCTTA
TCTGCCCTTAATCTGCAACTCTCTGTACAGTCTTCTACTTCATAA

Structures

Toxin

• AlphaFold DB: I3BGL7

Antitoxin

• AlphaFold DB: I3BGP0