Detailed information of TA system TA177317

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2701249..2701988
• Replicon: chromosome
• Accession: NZ_CP062916
• Organism: Raoultella terrigena strain Res13-Abat-PEB01-P1-04-A

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: IMO34_RS12800
• Protein ID: WP_195711410.1
• Coordinates: 2701503..2701988 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A085GLQ4
• Locus tag: IMO34_RS12795
• Protein ID: WP_032611697.1
• Coordinates: 2701249..2701515 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMO34_RS12775	2696431..2697531	-	1101	WP_141334603.1	amino acid ABC transporter permease	-
IMO34_RS12780	2697547..2698725	-	1179	WP_041145579.1	amino acid ABC transporter permease	-
IMO34_RS12785	2698791..2699816	-	1026	WP_041145578.1	amino acid ABC transporter substrate-binding protein	-
IMO34_RS12790	2700508..2701110	+	603	WP_148249943.1	DJ-1/PfpI family protein	-
IMO34_RS12795	2701249..2701515	+	267	WP_032611697.1	DUF1778 domain-containing protein	Antitoxin
IMO34_RS12800	2701503..2701988	+	486	WP_195711410.1	GNAT family N-acetyltransferase	Toxin
IMO34_RS12805	2702367..2703107	+	741	WP_195711412.1	amino acid ABC transporter ATP-binding protein	-
IMO34_RS12810	2703118..2703816	+	699	WP_195711414.1	ABC transporter permease subunit	-
IMO34_RS12815	2703826..2704581	+	756	WP_195711416.1	amino acid ABC transporter permease	-
IMO34_RS12820	2704568..2705413	+	846	WP_115192723.1	transporter substrate-binding domain-containing protein	-
IMO34_RS12825	2705436..2705957	+	522	WP_195711418.1	L-2-amino-thiazoline-4-carboxylic acid hydrolase	-
IMO34_RS12830	2705957..2706709	+	753	WP_195711420.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	mgtB	2665918..2921949	256031

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (28-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17698.48 Da
• Isoelectric Point: 9.1188

>T177317 WP_195711410.1 NZ_CP062916:2701503-2701988 [Raoultella terrigena]
VGHVTAPEPLSAFHQVAEFVSGETVLDDWLKHKGLKNQALGAARTFVVCQKDTKQIAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSLRGIGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQIQPRTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T177317 NZ_CP083442:3207506-3207934 [Pseudomonas chlororaphis]
ATGATAGTACTCGACACAAATGTGTTGTCAGAACTGATGCGCCCTCAACCGGATACGGCGGTATTGCTGTGGATCGAAGC
GCAACCCGTCGAGGACCTCTACATCAGCGCTATGACCATGGCCGAGATTCTCCACGGCATCGCGCGCCTGCCCCACGGCA
AGCGCAAACAGACGCTGCATGCATCGGCGCTGGCCATGTTCGAGGAAGACTTCATCGACCGGATCGTGCCCTTCGGAACC
CAAGCGGCTCTTTACTACGCGCATCTGGTCTCGCATCGCGAAAGATCAGGTCAGCCGATCAACCTGGTGGATACGCAAAT
TGCAGCGATATGCCGGGTCCATGACGCCGCGATTGCCACCCGCAACACCAAGGATTTCACCGACACTGGGCTGATCGTTA
TCAACCCCTGGCTACCGCTGGAAATCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10164.64 Da
• Isoelectric Point: 4.3837

>AT177317 WP_032611697.1 NZ_CP062916:2701249-2701515 [Raoultella terrigena]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPIIEKLL
ARKPQWDM

Nucleotide

• Length: 267 bp

>AT177317 NZ_CP083442:3207264-3207509 [Pseudomonas chlororaphis]
ATGGCCAGCCTTACGATCCGCAATCTGGACGACCACATCAAAGAACGCCTGCGCATCGAGGCCGCTCACAATGGTCATTC
CATGGAAGAAGAAGCCCGATTGATTTTGCGCCGCGCATTGAGCAGGACAGCTGACAGCCAAGGCCTGGGCAGCCGTATCA
GGGCACGCTTTGCCGAGACCGGCGGAGTAGAGTTGGAGCTGCCTGAACGTACCGAACCGGCACGCTTGGTGGACCTGGGC
GAATGA

Structures

Antitoxin

• AlphaFold DB: A0A085GLQ4