Detailed information of TA system TA177308

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1860571..1861161
• Replicon: chromosome
• Accession: NZ_CP062916
• Organism: Raoultella terrigena strain Res13-Abat-PEB01-P1-04-A

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: IMO34_RS08650
• Protein ID: WP_195710928.1
• Coordinates: 1860829..1861161 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: IMO34_RS08645
• Protein ID: WP_195710918.1
• Coordinates: 1860571..1860828 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMO34_RS08615	1856146..1856892	-	747	WP_195710913.1	NAD(P)-binding domain-containing protein	-
IMO34_RS08620	1856994..1857362	+	369	WP_115192347.1	helix-turn-helix transcriptional regulator	-
IMO34_RS08625	1857574..1858578	+	1005	WP_000427623.1	IS110-like element IS4321 family transposase	-
IMO34_RS08630	1859115..1859747	+	633	WP_195710915.1	thymidylate kinase	-
IMO34_RS08635	1859813..1860019	+	207	WP_195710917.1	hypothetical protein	-
IMO34_RS08640	1860016..1860222	+	207	Protein_1685	helix-turn-helix domain-containing protein	-
IMO34_RS08645	1860571..1860828	+	258	WP_195710918.1	antitoxin	Antitoxin
IMO34_RS08650	1860829..1861161	+	333	WP_195710928.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IMO34_RS08660	1861622..1862539	+	918	WP_041146297.1	nitrogen assimilation transcriptional regulator NAC	-
IMO34_RS08665	1862646..1863596	+	951	WP_041146296.1	HTH-type transcriptional regulator Cbl	-
IMO34_RS08670	1863711..1864652	+	942	WP_041147688.1	L,D-transpeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1857574..1858578	1004

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (5-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11835.72 Da
• Isoelectric Point: 10.7239

>T177308 WP_195710928.1 NZ_CP062916:1860829-1861161 [Raoultella terrigena]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSRASFNKLTRLPVVVPITSGGNFARTAGFTVSLEGAGTKTIGVIRCDQPRTV
DMAARNGKRLERIPDAVVNEVLARLDAILS

Nucleotide

• Length: 333 bp

>T177308 NZ_CP083436:c1247553-1246807 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9363.86 Da
• Isoelectric Point: 4.4475

>AT177308 WP_195710918.1 NZ_CP062916:1860571-1860828 [Raoultella terrigena]
MYITRLKKVGGSVMLAVPPAVLKTLALSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT177308 NZ_CP083436:c1246806-1246636 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAAAAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA