Detailed information of TA system TA177305

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 52206..52753
• Replicon: plasmid unnamed973
• Accession: NZ_CP062915
• Organism: Escherichia coli O112ab:H8 strain Res13-Lact-EA06-22

Toxin (Protein)

• Gene name: parE
• Uniprot ID: E9LLH3
• Locus tag: IMP05_RS25520
• Protein ID: WP_000969996.1
• Coordinates: 52206..52487 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: IMP05_RS25525
• Protein ID: WP_000079941.1
• Coordinates: 52484..52753 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP05_RS25485	47514..47783	+	270	WP_000379710.1	SemiSWEET transporter	-
IMP05_RS25490	47780..48046	+	267	WP_001017350.1	NAD(P)/FAD-dependent oxidoreductase	-
IMP05_RS25495	48102..48554	-	453	WP_000670963.1	N-acetyltransferase	-
IMP05_RS25500	48847..49107	+	261	WP_000343091.1	hypothetical protein	-
IMP05_RS25505	49104..49694	+	591	WP_001350923.1	hypothetical protein	-
IMP05_RS25510	49714..49932	-	219	WP_024133202.1	hypothetical protein	-
IMP05_RS25515	49980..52160	-	2181	WP_195717862.1	IS66 family transposase	-
IMP05_RS25520	52206..52487	-	282	WP_000969996.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IMP05_RS25525	52484..52753	-	270	WP_000079941.1	hypothetical protein	Antitoxin
IMP05_RS25530	53669..54526	-	858	WP_000130940.1	incFII family plasmid replication initiator RepA	-
IMP05_RS25535	54519..54593	-	75	WP_001365705.1	RepA leader peptide Tap	-
IMP05_RS25540	54832..55086	-	255	WP_000083830.1	replication regulatory protein RepA	-
IMP05_RS25545	55408..56244	-	837	WP_000480968.1	aminoglycoside O-phosphotransferase APH(6)-Id	-
IMP05_RS25550	56244..57047	-	804	WP_001082319.1	aminoglycoside O-phosphotransferase APH(3'')-Ib	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(6)-Id / aph(3'')-Ib / tet(B) / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..151873	151873
	-	inside	IScluster/Tn	aph(6)-Id / aph(3'')-Ib / tet(B)	-	44689..68249	23560

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10893.57 Da
• Isoelectric Point: 8.4615

>T177305 WP_000969996.1 NZ_CP062915:c52487-52206 [Escherichia coli O112ab:H8]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T177305 NZ_CP083434:2585416-2585778 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT177305 WP_000079941.1 NZ_CP062915:c52753-52484 [Escherichia coli O112ab:H8]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT177305 NZ_CP083434:2585249-2585419 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Toxin

• AlphaFold DB: E9LLH3

Antitoxin

• AlphaFold DB: A0A629TXC5