Detailed information of TA system TA177304

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 39008..39384
• Replicon: plasmid unnamed973
• Accession: NZ_CP062915
• Organism: Escherichia coli O112ab:H8 strain Res13-Lact-EA06-22

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A075MDK3
• Locus tag: IMP05_RS25430
• Protein ID: WP_000969988.1
• Coordinates: 39008..39190 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: IMP05_RS25435
• Protein ID: WP_001324224.1
• Coordinates: 39187..39384 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP05_RS25420	37007..38041	-	1035	WP_001190234.1	3-deoxy-7-phosphoheptulonate synthase	-
IMP05_RS25425	38601..38909	-	309	WP_000377483.1	hypothetical protein	-
IMP05_RS25430	39008..39190	-	183	WP_000969988.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IMP05_RS25435	39187..39384	-	198	WP_001324224.1	toxin-antitoxin system protein	Antitoxin
IMP05_RS25440	40066..41340	+	1275	WP_000489608.1	HlyD family secretion protein	-
IMP05_RS25445	41315..43429	+	2115	WP_001183604.1	peptidase domain-containing ABC transporter	-
IMP05_RS25450	43599..43910	-	312	WP_001259758.1	colicin V	-
IMP05_RS25455	43888..44124	-	237	WP_001323890.1	colicin V immunity protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(6)-Id / aph(3'')-Ib / tet(B) / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..151873	151873

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6931.07 Da
• Isoelectric Point: 4.5349

>T177304 WP_000969988.1 NZ_CP062915:c39190-39008 [Escherichia coli O112ab:H8]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGCSASLLFSLSFISVQVHDNIITV

Nucleotide

• Length: 183 bp

>T177304 NZ_CP083434:2565829-2566038 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGACGGTCAAGTAACTGTATCGTATGAAACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7658.53 Da
• Isoelectric Point: 7.2046

>AT177304 WP_001324224.1 NZ_CP062915:c39384-39187 [Escherichia coli O112ab:H8]
MDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK

Nucleotide

• Length: 198 bp

>AT177304 NZ_CP083434:2565524-2565817 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• AlphaFold DB: A0A075MDK3