Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataT-KacA/DUF1778(antitoxin)
Location 34090..34888 Replicon plasmid unnamed920
Accession NZ_CP062914
Organism Escherichia coli O112ab:H8 strain Res13-Lact-EA06-22

Toxin (Protein)


Gene name ataT Uniprot ID E2QDF3
Locus tag IMP05_RS24815 Protein ID WP_000072677.1
Coordinates 34367..34888 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A0V9R6Q7
Locus tag IMP05_RS24810 Protein ID WP_001351987.1
Coordinates 34090..34359 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP05_RS24785 29517..30857 - 1341 WP_000137333.1 AAA family ATPase -
IMP05_RS24790 30901..31641 - 741 WP_001717320.1 hypothetical protein -
IMP05_RS24795 31924..32691 + 768 WP_000342417.1 hypothetical protein -
IMP05_RS24800 32744..33097 - 354 WP_160394000.1 hypothetical protein -
IMP05_RS24805 33103..33771 - 669 WP_000161228.1 AAA family ATPase -
IMP05_RS24810 34090..34359 + 270 WP_001351987.1 DUF1778 domain-containing protein Antitoxin
IMP05_RS24815 34367..34888 + 522 WP_000072677.1 GNAT family N-acetyltransferase Toxin
IMP05_RS24820 35057..35308 - 252 WP_000901559.1 hypothetical protein -
IMP05_RS24825 35310..36002 - 693 WP_032084069.1 membrane protein -
IMP05_RS24830 36016..36339 - 324 WP_032084070.1 hypothetical protein -
IMP05_RS24835 36414..37196 - 783 WP_072647505.1 receptor-recognizing protein -
IMP05_RS24840 37262..38956 - 1695 Protein_40 tail fiber domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sitABCD - 1..120310 120310


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19392.17 Da        Isoelectric Point: 8.6369

>T177303 WP_000072677.1 NZ_CP062914:34367-34888 [Escherichia coli O112ab:H8]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLANHLKRQHEGKILRAYVLCTQEERPKVLGYYTLSGSCFEKESLPSRSKQ
KKVPYRNVPSVTLGRLALDKSLQGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLGFIQLVGNNERSL
FYPTKSIEKLFEE

Download         Length: 522 bp

>T177303 NZ_CP083434:2505949-2506053 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTGATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10082.54 Da        Isoelectric Point: 7.0155

>AT177303 WP_001351987.1 NZ_CP062914:34090-34359 [Escherichia coli O112ab:H8]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKR
AADRLKSME

Download         Length: 270 bp

>AT177303 NZ_CP083434:c2506212-2506048 [Staphylococcus aureus]
GTAGTAAGTAGAAGCAAAAGATGAAAATCTTTAACTCTTGAAACACAAAAAGGGCAACACTCGGAAACATGTTACCCTAA
TGAGCCCGTTAAAAAGACGGTGACCTTATATTTTATTTAAAAATAGCCTTCAAAAATGCCGGTCAAAGCGAATAGAAGGT
TATTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9R6P3

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9R6Q7

References