Detailed information of TA system TA177297

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4542387..4543012
• Replicon: chromosome
• Accession: NZ_CP062910
• Organism: Escherichia coli O112ab:H8 strain Res13-Lact-EA06-22

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IMP05_RS21920
• Protein ID: WP_000911330.1
• Coordinates: 4542614..4543012 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: IMP05_RS21915
• Protein ID: WP_000450524.1
• Coordinates: 4542387..4542614 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP05_RS21890	4538190..4538660	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
IMP05_RS21895	4538660..4539232	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
IMP05_RS21900	4539378..4540256	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
IMP05_RS21905	4540273..4541307	+	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
IMP05_RS21910	4541520..4542233	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
IMP05_RS21915	4542387..4542614	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
IMP05_RS21920	4542614..4543012	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
IMP05_RS21925	4543159..4544022	+	864	Protein_4272	neutral zinc metallopeptidase	-
IMP05_RS21930	4544037..4546052	+	2016	WP_047086710.1	tRNA cytosine(34) acetyltransferase TmcA	-
IMP05_RS21935	4546126..4546824	+	699	WP_000679812.1	esterase	-
IMP05_RS21940	4546934..4547134	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T177297 WP_000911330.1 NZ_CP062910:4542614-4543012 [Escherichia coli O112ab:H8]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T177297 NZ_CP083410:c4579704-4579363 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT177297 WP_000450524.1 NZ_CP062910:4542387-4542614 [Escherichia coli O112ab:H8]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT177297 NZ_CP083410:4579699-4579775 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Antitoxin

• AlphaFold DB: A0A829CM33