Detailed information of TA system TA177290

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2242951..2243749
• Replicon: chromosome
• Accession: NZ_CP062910
• Organism: Escherichia coli O112ab:H8 strain Res13-Lact-EA06-22

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q8VRA6
• Locus tag: IMP05_RS11020
• Protein ID: WP_000854730.1
• Coordinates: 2243372..2243749 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0P0SQV8
• Locus tag: IMP05_RS11015
• Protein ID: WP_001285481.1
• Coordinates: 2242951..2243325 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP05_RS10975	2238926..2239378	+	453	WP_000682723.1	hypothetical protein	-
IMP05_RS10980	2239496..2239729	+	234	WP_001213776.1	DUF905 family protein	-
IMP05_RS10985	2239829..2240650	+	822	WP_001234359.1	DUF945 domain-containing protein	-
IMP05_RS10990	2240650..2240895	+	246	WP_001164966.1	hypothetical protein	-
IMP05_RS10995	2240989..2241462	+	474	WP_001313575.1	antirestriction protein	-
IMP05_RS11000	2241478..2241954	+	477	WP_001313574.1	RadC family protein	-
IMP05_RS11005	2242017..2242238	+	222	WP_000692315.1	DUF987 domain-containing protein	-
IMP05_RS11010	2242257..2242901	+	645	WP_000086759.1	hypothetical protein	-
IMP05_RS11015	2242951..2243325	+	375	WP_001285481.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
IMP05_RS11020	2243372..2243749	+	378	WP_000854730.1	TA system toxin CbtA family protein	Toxin
IMP05_RS11025	2243746..2244238	+	493	Protein_2164	hypothetical protein	-
IMP05_RS11030	2244317..2245305	-	989	Protein_2165	IS630 family transposase	-
IMP05_RS11035	2245453..2247046	-	1594	Protein_2166	IS66 family transposase	-
IMP05_RS11040	2247077..2247427	-	351	WP_000624707.1	IS66 family insertion sequence element accessory protein TnpB	-
IMP05_RS11045	2247424..2247715	-	292	Protein_2168	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14212.21 Da
• Isoelectric Point: 7.2923

>T177290 WP_000854730.1 NZ_CP062910:2243372-2243749 [Escherichia coli O112ab:H8]
MKTLPDTHVREASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRSNYRMVNDIIRGEHSEAKR

Nucleotide

• Length: 378 bp

>T177290 NZ_CP083410:c3168406-3168110 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13817.53 Da
• Isoelectric Point: 4.7511

>AT177290 WP_001285481.1 NZ_CP062910:2242951-2243325 [Escherichia coli O112ab:H8]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTIG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT177290 NZ_CP083410:c3168108-3167713 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: A0A0H2V671

Antitoxin

• AlphaFold DB: A0A0P0SQV8