Detailed information of TA system TA177289

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1902188..1903025
• Replicon: chromosome
• Accession: NZ_CP062910
• Organism: Escherichia coli O112ab:H8 strain Res13-Lact-EA06-22

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: IMP05_RS09400
• Protein ID: WP_063120031.1
• Coordinates: 1902483..1903025 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: IMP05_RS09395
• Protein ID: WP_001297137.1
• Coordinates: 1902188..1902499 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP05_RS09370	1897208..1898155	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
IMP05_RS09375	1898177..1900168	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
IMP05_RS09380	1900158..1900772	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
IMP05_RS09385	1900772..1901101	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
IMP05_RS09390	1901113..1902003	+	891	WP_000971336.1	heme o synthase	-
IMP05_RS09395	1902188..1902499	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
IMP05_RS09400	1902483..1903025	+	543	WP_063120031.1	GNAT family N-acetyltransferase	Toxin
IMP05_RS09405	1903081..1904016	-	936	WP_001297127.1	sel1 repeat family protein	-
IMP05_RS09410	1904423..1905787	+	1365	WP_001000978.1	MFS transporter	-
IMP05_RS09415	1905915..1906406	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
IMP05_RS09420	1906574..1907485	+	912	WP_000705853.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19705.88 Da
• Isoelectric Point: 8.0330

>T177289 WP_063120031.1 NZ_CP062910:1902483-1903025 [Escherichia coli O112ab:H8]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVAGKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T177289 NZ_CP083410:c3040685-3040278 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT177289 WP_001297137.1 NZ_CP062910:1902188-1902499 [Escherichia coli O112ab:H8]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT177289 NZ_CP083410:c3040932-3040666 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0Y6B3