Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1868112..1868730 Replicon chromosome
Accession NZ_CP062910
Organism Escherichia coli O112ab:H8 strain Res13-Lact-EA06-22

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag IMP05_RS09230 Protein ID WP_001291435.1
Coordinates 1868512..1868730 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag IMP05_RS09225 Protein ID WP_000344800.1
Coordinates 1868112..1868486 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP05_RS09215 1863201..1864394 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
IMP05_RS09220 1864417..1867566 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
IMP05_RS09225 1868112..1868486 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
IMP05_RS09230 1868512..1868730 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
IMP05_RS09235 1868901..1869452 + 552 WP_000102564.1 maltose O-acetyltransferase -
IMP05_RS09240 1869568..1870038 + 471 WP_000136192.1 YlaC family protein -
IMP05_RS09245 1870202..1871752 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
IMP05_RS09250 1871794..1872147 - 354 WP_000878140.1 DUF1428 domain-containing protein -
IMP05_RS09260 1872526..1872837 + 312 WP_000409911.1 MGMT family protein -
IMP05_RS09265 1872868..1873440 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T177288 WP_001291435.1 NZ_CP062910:1868512-1868730 [Escherichia coli O112ab:H8]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T177288 NZ_CP083410:c2910954-2910619 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT177288 WP_000344800.1 NZ_CP062910:1868112-1868486 [Escherichia coli O112ab:H8]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT177288 NZ_CP083410:c2911202-2910954 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References