Detailed information of TA system TA177287

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1203864..1204665
• Replicon: chromosome
• Accession: NZ_CP062910
• Organism: Escherichia coli O112ab:H8 strain Res13-Lact-EA06-22

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0H2V687
• Locus tag: IMP05_RS06205
• Protein ID: WP_000854739.1
• Coordinates: 1203864..1204244 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0P7R0L9
• Locus tag: IMP05_RS06210
• Protein ID: WP_001285482.1
• Coordinates: 1204291..1204665 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP05_RS06170	1199455..1200009	-	555	WP_001001902.1	molecular chaperone YcdY	-
IMP05_RS06175	1200033..1200770	-	738	WP_000283664.1	zinc-binding phosphatase	-
IMP05_RS06180	1200825..1201763	-	939	WP_000351317.1	glyoxylate/hydroxypyruvate reductase GhrA	-
IMP05_RS06190	1202250..1203077	-	828	WP_001304477.1	DUF4942 domain-containing protein	-
IMP05_RS06195	1203162..1203359	-	198	WP_000772027.1	DUF957 domain-containing protein	-
IMP05_RS06200	1203379..1203867	-	489	WP_001054232.1	hypothetical protein	-
IMP05_RS06205	1203864..1204244	-	381	WP_000854739.1	TA system toxin CbtA family protein	Toxin
IMP05_RS06210	1204291..1204665	-	375	WP_001285482.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
IMP05_RS06215	1204715..1205359	-	645	WP_000086763.1	hypothetical protein	-
IMP05_RS06220	1205378..1205599	-	222	WP_000692315.1	DUF987 domain-containing protein	-
IMP05_RS06225	1205662..1206138	-	477	WP_001186774.1	RadC family protein	-
IMP05_RS06230	1206154..1206627	-	474	WP_062826774.1	antirestriction protein	-
IMP05_RS06235	1206890..1207711	-	822	WP_001234710.1	DUF945 domain-containing protein	-
IMP05_RS06240	1207792..1208003	+	212	Protein_1228	hypothetical protein	-
IMP05_RS06245	1208122..1208577	-	456	WP_000581502.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14214.14 Da
• Isoelectric Point: 6.4773

>T177287 WP_000854739.1 NZ_CP062910:c1204244-1203864 [Escherichia coli O112ab:H8]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGVPSQLINSIDILRARRATGLMTRDNYRTVNDITLGRHPEEAKQ

Nucleotide

• Length: 381 bp

>T177287 NZ_CP083410:2777316-2777645 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13791.44 Da
• Isoelectric Point: 4.7511

>AT177287 WP_001285482.1 NZ_CP062910:c1204665-1204291 [Escherichia coli O112ab:H8]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT177287 NZ_CP083410:2776978-2777295 [Escherichia coli]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Structures

Toxin

• AlphaFold DB: A0A0H2V687

Antitoxin

• AlphaFold DB: A0A0P7R0L9