177276

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 28421..29064 Replicon plasmid unnamednovel_1
Accession NZ_CP062909
Organism Escherichia coli strain Res13-Lact-EB12-05

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag IMP23_RS24085 Protein ID WP_000754566.1
Coordinates 28421..28837 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag IMP23_RS24090 Protein ID WP_001261276.1
Coordinates 28834..29064 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP23_RS24055 24222..24728 + 507 WP_034167704.1 poly-beta-1,6-N-acetyl-D-glucosamine biosynthesis protein PgaD -
IMP23_RS24060 24809..24904 + 96 Protein_24 (4Fe-4S)-binding protein -
IMP23_RS24065 24968..25672 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
IMP23_RS24070 25730..26287 + 558 WP_001235700.1 recombinase family protein -
IMP23_RS24075 26387..26788 - 402 WP_096171525.1 hypothetical protein -
IMP23_RS24080 27360..28216 - 857 Protein_28 IS3 family transposase -
IMP23_RS24085 28421..28837 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
IMP23_RS24090 28834..29064 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
IMP23_RS24095 29263..29754 - 492 Protein_31 IS5-like element ISKpn26 family transposase -
IMP23_RS24100 29860..30564 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
IMP23_RS24105 30618..30827 + 210 Protein_33 IS110 family transposase -
IMP23_RS24110 31104..31579 - 476 Protein_34 IS1 family transposase -
IMP23_RS24115 31973..33001 + 1029 WP_063097364.1 tyrosine-type recombinase/integrase -
IMP23_RS24120 33024..33914 - 891 Protein_36 IS30-like element IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..49398 49398
- inside IScluster/Tn - - 24968..45409 20441


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T177276 WP_000754566.1 NZ_CP062909:c28837-28421 [Escherichia coli]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T177276 NZ_CP083410:c1269810-1269703 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT177276 WP_001261276.1 NZ_CP062909:c29064-28834 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT177276 NZ_CP083410:1269857-1269923 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References