Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4275839..4276336 Replicon chromosome
Accession NZ_CP062908
Organism Escherichia coli strain Res13-Lact-EB12-05

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag IMP23_RS21780 Protein ID WP_001173343.1
Coordinates 4275839..4276012 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag IMP23_RS21785 Protein ID WP_000398619.1
Coordinates 4276040..4276336 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP23_RS21770 4272509..4273966 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
IMP23_RS21775 4274203..4275720 + 1518 WP_001295074.1 lysine--tRNA ligase -
IMP23_RS21780 4275839..4276012 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
IMP23_RS21785 4276040..4276336 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
IMP23_RS21790 4276563..4276835 - 273 WP_000405651.1 N-acetyltransferase -
IMP23_RS21795 4276847..4277077 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
IMP23_RS21800 4277258..4278889 + 1632 WP_001216477.1 sensor histidine kinase -
IMP23_RS21805 4278886..4279605 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T177275 WP_001173343.1 NZ_CP062908:c4276012-4275839 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T177275 NZ_CP083410:c1269275-1269168 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT177275 WP_000398619.1 NZ_CP062908:c4276336-4276040 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT177275 NZ_CP083410:1269323-1269389 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References