Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4018051..4018627 Replicon chromosome
Accession NZ_CP062908
Organism Escherichia coli strain Res13-Lact-EB12-05

Toxin (Protein)


Gene name shpA Uniprot ID A0A0E2LC92
Locus tag IMP23_RS20530 Protein ID WP_001299718.1
Coordinates 4018340..4018627 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A0E2KXJ8
Locus tag IMP23_RS20525 Protein ID WP_000063159.1
Coordinates 4018051..4018353 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP23_RS20510 4014640..4016790 + 2151 WP_001299714.1 pyruvate/proton symporter BtsT -
IMP23_RS20515 4016840..4017043 + 204 WP_000467859.1 YbdD/YjiX family protein -
IMP23_RS20520 4017054..4018010 + 957 WP_001299717.1 GTPase -
IMP23_RS20525 4018051..4018353 - 303 WP_000063159.1 BrnA antitoxin family protein Antitoxin
IMP23_RS20530 4018340..4018627 - 288 WP_001299718.1 BrnT family toxin Toxin
IMP23_RS20535 4018824..4019990 + 1167 Protein_3781 restriction endonuclease -
IMP23_RS20540 4020054..4021673 + 1620 WP_001029740.1 type I restriction-modification system subunit M -
IMP23_RS20545 4021663..4023054 + 1392 WP_000535334.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11142.60 Da        Isoelectric Point: 7.4697

>T177273 WP_001299718.1 NZ_CP062908:c4018627-4018340 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGVVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T177273 NZ_CP083410:c263669-263328 [Escherichia coli]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11536.01 Da        Isoelectric Point: 9.4646

>AT177273 WP_000063159.1 NZ_CP062908:c4018353-4018051 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEEIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT177273 NZ_CP083410:c264007-263690 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E2LC92

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2KXJ8

References