Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1323277..1323902 Replicon chromosome
Accession NZ_CP062901
Organism Escherichia coli O152:H23 strain Res13-Lact-ER01-35

Toxin (Protein)


Gene name vapC Uniprot ID A0A2J1DF47
Locus tag IMP37_RS06345 Protein ID WP_000911331.1
Coordinates 1323504..1323902 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag IMP37_RS06340 Protein ID WP_000450524.1
Coordinates 1323277..1323504 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP37_RS06315 1319080..1319550 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
IMP37_RS06320 1319550..1320122 - 573 WP_000176191.1 glycine cleavage system transcriptional repressor -
IMP37_RS06325 1320268..1321146 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
IMP37_RS06330 1321163..1322197 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
IMP37_RS06335 1322410..1323123 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
IMP37_RS06340 1323277..1323504 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
IMP37_RS06345 1323504..1323902 + 399 WP_000911331.1 tRNA(fMet)-specific endonuclease VapC Toxin
IMP37_RS06350 1324049..1324912 + 864 WP_001267507.1 neutral zinc metallopeptidase -
IMP37_RS06355 1324927..1326942 + 2016 WP_000829332.1 tRNA cytosine(34) acetyltransferase TmcA -
IMP37_RS06360 1327016..1327714 + 699 WP_000679823.1 esterase -
IMP37_RS06365 1327795..1327995 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14862.19 Da        Isoelectric Point: 9.2216

>T177226 WP_000911331.1 NZ_CP062901:1323504-1323902 [Escherichia coli O152:H23]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLVELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T177226 NZ_CP083351:c142327-142046 [Ensifer adhaerens]
GTGATCAGGTCATTCAAGGACGCTAAAACCGAAGCCGTTGCCCGCGGCAAAGCACCAAAGGGCTTTCCTTCGGACATCGT
GAGGTCGGCCGTCCGGAAATTGACAATCCTGAACAACGCTGCAGCTCTTGAGGACTTGCGTTCTCCTCCGGGCAATCGAT
TGGAAGCCCTTAAGGGGAATAGAGAAGGCCAACATTCGATCCGCATCAATGATCAGTGGCGCATATGCTTCGTTTGGACT
GATGCCGGTATCGAAGACGTTGAAATAGTTGACTACCATTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT177226 WP_000450524.1 NZ_CP062901:1323277-1323504 [Escherichia coli O152:H23]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT177226 NZ_CP083351:c142015-141716 [Ensifer adhaerens]
ATGACCCATATTCTTCCGCCGGTCCACCCGGGCGAAATCCTCCGCGAGGAGTATCTGCTCCCCCTGAACATGAGTGCCGG
TGCACTGGCGAAAAAGCTCAATGTTCCTCGCACCCGCATCGAACGTATAGCGGCGGAGCAGACACCGATAACGACGGATA
CCGCCCTCCGGCTCGCCAAGTACTTCCGCACAACGCCGGAATTTTGGATGAACATGCAGACCAGCTTCAACCTCAAGACA
GAGGAAGTGGCGCTTCATAGCGAACTGGAATTGATCCCCGAAATCGACAGAGCAGCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J1DF47

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References