Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1029112..1029695 Replicon chromosome
Accession NZ_CP062901
Organism Escherichia coli O152:H23 strain Res13-Lact-ER01-35

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag IMP37_RS04930 Protein ID WP_000254745.1
Coordinates 1029360..1029695 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag IMP37_RS04925 Protein ID WP_000581937.1
Coordinates 1029112..1029360 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP37_RS04915 1025451..1026752 + 1302 WP_000046785.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
IMP37_RS04920 1026800..1029034 + 2235 WP_000226815.1 GTP pyrophosphokinase -
IMP37_RS04925 1029112..1029360 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
IMP37_RS04930 1029360..1029695 + 336 WP_000254745.1 endoribonuclease MazF Toxin
IMP37_RS04935 1029766..1030557 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
IMP37_RS04940 1030785..1032422 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
IMP37_RS04945 1032510..1033808 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T177225 WP_000254745.1 NZ_CP062901:1029360-1029695 [Escherichia coli O152:H23]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T177225 NZ_CP083351:131026-131445 [Ensifer adhaerens]
ATGATCGTTCTGGACACAAATGTCGTCTCGGAAGCGATGAAGCCGGCCCCGGATTTAGCCGTGCGTAATTGGTTAAATGA
TCAAGTGGCTGAAACCTTGTATCTGTCCAGTGTGACGCTGGCGGAGTTACTCTTTGGCATTGCTGCGCTGCCGGAGGGAC
GGCGCAAAAAGGCGTTGGCCGAAACTCTTGATGGTATGCTCGAACTGTTTGATGATCGCGTGTTGCCATTCGACACTGCA
GCCGCTCGGCACTATGCCGACCTTGCGGCAATCGCGCGAGCGGCGGGGAAGGGATTTCCGACACCTGATGGTTACATTGT
CGCTATCGCAGCCTCCAGGGGGTTTATAATCGCAACACGCGATACAAGCCCTTTTGAGGCGGCAGGTCTCTCCGTCGTAA
ATCCTTGGAGTGACCTATAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT177225 WP_000581937.1 NZ_CP062901:1029112-1029360 [Escherichia coli O152:H23]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT177225 NZ_CP083351:130775-131029 [Ensifer adhaerens]
ATGGCGATCCTGACGGTACGAAATGTGCCTGATGAAGTGCACCGCGCTTTGCGTGTCCGGGCGGCGATTCACGGCCGCAG
CACAGAGGCGGAAGTCCGCGAGATTTTGGAACGTGCGGTCAAGCCCGAACAGCGTGTCCGCATAGGCGACGCCTTGGCTG
AGCTGAGCGGGCGGCTTGGCCTGACCAATGAGGATTTTGAAGTGTTCGACCAGGTGCGAGACAAAGTGCCGGCTGAGCCA
ATGAGGTTTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References