Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 279780..280580 Replicon chromosome
Accession NZ_CP062901
Organism Escherichia coli O152:H23 strain Res13-Lact-ER01-35

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag IMP37_RS01270 Protein ID WP_000342449.1
Coordinates 280053..280580 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag IMP37_RS01265 Protein ID WP_001277108.1
Coordinates 279780..280046 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP37_RS01245 275438..276106 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
IMP37_RS01250 276099..277157 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
IMP37_RS01255 277402..278256 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
IMP37_RS01260 278527..279630 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
IMP37_RS01265 279780..280046 + 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
IMP37_RS01270 280053..280580 + 528 WP_000342449.1 GNAT family N-acetyltransferase Toxin
IMP37_RS01275 280577..280960 - 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -
IMP37_RS01280 281384..282493 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
IMP37_RS01285 282541..283467 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
IMP37_RS01290 283464..284741 + 1278 WP_000803771.1 branched chain amino acid ABC transporter permease LivM -
IMP37_RS01295 284738..285505 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T177221 WP_000342449.1 NZ_CP062901:280053-280580 [Escherichia coli O152:H23]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T177221 NZ_CP083348:274851-275141 [Ensifer adhaerens]
ATGCCACAAATAGTCTTCTCTTCAGCGGCTGTTCGTGACCTAGAGCGTCTCCGAAAATACTTGCAGCCCAAAAGCCCTTT
GGCTGCCAAGCGAGCGGCTGAAGCCATCCTGAAGGGTGTGCAGGCCCTTGGCGCACATCCAAAAATGGGGCGCCTTATTG
ATGATCTGCCGGATCAGTATCGAGAGTGGCTCATTGATTTTGGAGATAGCGGATACATCACGCGTTACCGCATTGATGAG
GACGTTTTGACAATCCTCGCCGTGCGGCACCAGAAAGAAAGCGGCTTCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT177221 WP_001277108.1 NZ_CP062901:279780-280046 [Escherichia coli O152:H23]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT177221 NZ_CP083348:274585-274863 [Ensifer adhaerens]
ATGGCATCCCCAACCTCGCTCAAGCTCGATGATGAGCTGAAAGGTCGCGTTCAACACTTAGCTGAAATCCGCCGCCGTTC
GTCTCACTGGATCATGCGAGAAGCGATTGCGCAATACGTCGAGCGAGAAGAGAAGCGAGAAACATTGCGACAGGACACGC
TGGACGCGTGGGAGGAATATCAATCGGCGGGCCTCCATGCCACTGCGGCCGAGGTCGAAAAATGGCTATCCACGTGGGGA
ACTGACGACGAGAGCGCTGCGCCTGAATGCCACAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References