Detailed information of TA system TA177190

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1049685..1050268
• Replicon: chromosome
• Accession: NZ_CP062895
• Organism: Escherichia coli strain Res13-Lact-ER02-39

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IMP38_RS05035
• Protein ID: WP_113263827.1
• Coordinates: 1049933..1050268 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A1V2T5M9
• Locus tag: IMP38_RS05030
• Protein ID: WP_000581941.1
• Coordinates: 1049685..1049933 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP38_RS05020	1046024..1047325	+	1302	WP_000046812.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-
IMP38_RS05025	1047373..1049607	+	2235	WP_000226815.1	GTP pyrophosphokinase	-
IMP38_RS05030	1049685..1049933	+	249	WP_000581941.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IMP38_RS05035	1049933..1050268	+	336	WP_113263827.1	endoribonuclease MazF	Toxin
IMP38_RS05040	1050339..1051130	+	792	WP_001071648.1	nucleoside triphosphate pyrophosphohydrolase	-
IMP38_RS05045	1051358..1052995	+	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
IMP38_RS05050	1053083..1054381	+	1299	WP_000036723.1	phosphopyruvate hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12141.07 Da
• Isoelectric Point: 8.7218

>T177190 WP_113263827.1 NZ_CP062895:1049933-1050268 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQRIKAKINVLIG

Nucleotide

• Length: 336 bp

>T177190 NZ_CP083273:4357141-4357398 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9385.76 Da
• Isoelectric Point: 4.4665

>AT177190 WP_000581941.1 NZ_CP062895:1049685-1049933 [Escherichia coli]
MIHSSVKRWGNSPAVRIPTTLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT177190 NZ_CP083273:4357410-4357955 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1V2T5M9