Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 68239..68764 Replicon plasmid unnamed973
Accession NZ_CP062893
Organism Escherichia coli O100:H21 strain Res13-Lact-ER07-18

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag IMP41_RS23670 Protein ID WP_001159868.1
Coordinates 68239..68544 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag IMP41_RS23675 Protein ID WP_000813634.1
Coordinates 68546..68764 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP41_RS23655 64171..65337 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
IMP41_RS23660 65925..66680 - 756 WP_000852146.1 replication initiation protein RepE -
IMP41_RS23665 67432..68238 - 807 WP_000016982.1 site-specific integrase -
IMP41_RS23670 68239..68544 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
IMP41_RS23675 68546..68764 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
IMP41_RS23680 69332..69844 + 513 WP_000151784.1 hypothetical protein -
IMP41_RS23685 69878..71011 - 1134 WP_000545984.1 DUF3800 domain-containing protein -
IMP41_RS23690 71178..71951 - 774 WP_000905949.1 hypothetical protein -
IMP41_RS23695 71964..72464 - 501 WP_000528932.1 hypothetical protein -
IMP41_RS23700 72729..72959 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
IMP41_RS23705 72956..73372 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sitABCD iroB / iroC / iroN / iroE / iroD / iutA / iucD / iucC / iucB / iucA 1..99946 99946


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T177182 WP_001159868.1 NZ_CP062893:c68544-68239 [Escherichia coli O100:H21]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T177182 NZ_CP083273:c2626743-2626570 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT177182 WP_000813634.1 NZ_CP062893:c68764-68546 [Escherichia coli O100:H21]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT177182 NZ_CP083273:c2627067-2626771 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References