177175

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 61305..61906 Replicon plasmid unnamed1009
Accession NZ_CP062890
Organism Escherichia coli O100:H21 strain Res13-Lact-ER07-18

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag IMP41_RS22485 Protein ID WP_001216034.1
Coordinates 61305..61685 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag IMP41_RS22490 Protein ID WP_001190712.1
Coordinates 61685..61906 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP41_RS22460 56746..58230 - 1485 WP_000124150.1 terminase -
IMP41_RS22465 58230..59423 - 1194 WP_032153803.1 hypothetical protein -
IMP41_RS22470 59509..59961 - 453 WP_001326849.1 late promoter-activating protein -
IMP41_RS22475 60050..61093 - 1044 WP_032153800.1 DUF968 domain-containing protein -
IMP41_RS22480 61121..61300 - 180 WP_009446826.1 hypothetical protein -
IMP41_RS22485 61305..61685 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
IMP41_RS22490 61685..61906 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
IMP41_RS22495 61979..62368 - 390 WP_000506730.1 DNA repair protein -
IMP41_RS22500 62543..63127 + 585 WP_032272826.1 hypothetical protein -
IMP41_RS22505 63305..64002 - 698 Protein_63 IS1 family transposase -
IMP41_RS22510 64207..64680 - 474 WP_077632598.1 hypothetical protein -
IMP41_RS22515 65839..66017 - 179 Protein_65 hypothetical protein -
IMP41_RS22520 66019..66705 - 687 WP_072148508.1 ead/Ea22-like family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..89847 89847
- flank IS/Tn - - 63727..64002 275


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T177175 WP_001216034.1 NZ_CP062890:c61685-61305 [Escherichia coli O100:H21]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T177175 NZ_CP083267:2539400-2539654 [Streptomyces sp. A144]
GTGAGAGTCGCTTTCACGTCGCACGGCTGGGAGGACTACGTCCACTGGGCCGAGAGCGACCGGAAGATGACCAGACGGAT
CAACAAGCTGATCGCCGACATCGGGCGCGACCCCTTCAAGGGCATCGGCAAGCCGGAGCCGCTGAAGGGGGACCTGTCGG
GCTACTGGTCGCGGCGCATCGACGACACGCACCGTCTGGTGTACAAGCCGGGCGACGGCGAACTGGTCGTCGTCCAGGCG
CGGTACCACTACTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT177175 WP_001190712.1 NZ_CP062890:c61906-61685 [Escherichia coli O100:H21]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT177175 NZ_CP083267:2539140-2539403 [Streptomyces sp. A144]
ATGTCCATCACCGCCAGCGAAGCCCGTCAGAACCTCTTCCCGCTGATAGAGCAGGTCAACGAGGACCACGCCCCGGTGCA
CATCACCTCCCGCAAGGGGAACGCCGTGCTGATGTCCGAGGAGGACTTCACGGCGTGGACGGAGACGGTGCACCTGCTGC
GGTCGCCGAAGAACGCCCGCCGTCTGCTCGATTCGATCGCGGAGGCCGAGGCCGGCGAGGCACGGCACCGCGAGCTGATC
GACCCGGACGCGGAGCGGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References