Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4170852..4171349 Replicon chromosome
Accession NZ_CP062889
Organism Escherichia coli O100:H21 strain Res13-Lact-ER07-18

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag IMP41_RS20020 Protein ID WP_001173343.1
Coordinates 4170852..4171025 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag IMP41_RS20025 Protein ID WP_000398619.1
Coordinates 4171053..4171349 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP41_RS20010 4167522..4168979 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
IMP41_RS20015 4169216..4170733 + 1518 WP_001295074.1 lysine--tRNA ligase -
IMP41_RS20020 4170852..4171025 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
IMP41_RS20025 4171053..4171349 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
IMP41_RS20030 4171576..4171848 - 273 WP_000405649.1 N-acetyltransferase -
IMP41_RS20035 4171860..4172090 - 231 WP_000371700.1 (4Fe-4S)-binding protein -
IMP41_RS20040 4172271..4173902 + 1632 WP_001216487.1 sensor histidine kinase -
IMP41_RS20045 4173899..4174618 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T177173 WP_001173343.1 NZ_CP062889:c4171025-4170852 [Escherichia coli O100:H21]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T177173 NZ_CP083264:100669-100818 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCTACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT177173 WP_000398619.1 NZ_CP062889:c4171349-4171053 [Escherichia coli O100:H21]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT177173 NZ_CP083264:c100625-100564 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References