Detailed information of TA system TA177167

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3387203..3388040
• Replicon: chromosome
• Accession: NZ_CP062889
• Organism: Escherichia coli O100:H21 strain Res13-Lact-ER07-18

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: IMP41_RS16240
• Protein ID: WP_000227784.1
• Coordinates: 3387498..3388040 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: IMP41_RS16235
• Protein ID: WP_001297137.1
• Coordinates: 3387203..3387514 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP41_RS16210	3382223..3383170	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
IMP41_RS16215	3383192..3385183	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
IMP41_RS16220	3385173..3385787	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
IMP41_RS16225	3385787..3386116	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
IMP41_RS16230	3386128..3387018	+	891	WP_000971336.1	heme o synthase	-
IMP41_RS16235	3387203..3387514	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
IMP41_RS16240	3387498..3388040	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
IMP41_RS16245	3388096..3389031	-	936	WP_001365761.1	sel1 repeat family protein	-
IMP41_RS16250	3389439..3390803	+	1365	WP_001000974.1	MFS transporter	-
IMP41_RS16255	3390931..3391422	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
IMP41_RS16260	3391590..3392501	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T177167 WP_000227784.1 NZ_CP062889:3387498-3388040 [Escherichia coli O100:H21]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T177167 NZ_CP083263:4060591-4060998 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT177167 WP_001297137.1 NZ_CP062889:3387203-3387514 [Escherichia coli O100:H21]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT177167 NZ_CP083263:4060344-4060610 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3