Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2368094..2368732 Replicon chromosome
Accession NZ_CP062889
Organism Escherichia coli O100:H21 strain Res13-Lact-ER07-18

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag IMP41_RS11375 Protein ID WP_000813794.1
Coordinates 2368556..2368732 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag IMP41_RS11370 Protein ID WP_001270286.1
Coordinates 2368094..2368510 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP41_RS11350 2363246..2364187 - 942 WP_001251315.1 ABC transporter permease -
IMP41_RS11355 2364188..2365201 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
IMP41_RS11360 2365219..2366364 - 1146 WP_000047456.1 ABC transporter substrate-binding protein -
IMP41_RS11365 2366609..2368015 - 1407 WP_000760592.1 PLP-dependent aminotransferase family protein -
IMP41_RS11370 2368094..2368510 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
IMP41_RS11375 2368556..2368732 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
IMP41_RS11380 2368954..2369184 + 231 WP_000494244.1 YncJ family protein -
IMP41_RS11385 2369276..2371237 - 1962 WP_001593323.1 23S rRNA 5-hydroxycytidine C2501 synthase -
IMP41_RS11390 2371310..2371846 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
IMP41_RS11395 2371899..2373113 + 1215 WP_001348055.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T177165 WP_000813794.1 NZ_CP062889:c2368732-2368556 [Escherichia coli O100:H21]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T177165 NZ_CP083263:c3172753-3172376 [Escherichia coli]
ATGAACACATTACCCGATACACACGTACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACTCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT177165 WP_001270286.1 NZ_CP062889:c2368510-2368094 [Escherichia coli O100:H21]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT177165 NZ_CP083263:c3173199-3172843 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCTGCGAAGCCGACACCCTCGGCAGTTGTGGCTACGTTTA
TATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References