Detailed information of TA system TA177154

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1280483..1281108
• Replicon: chromosome
• Accession: NZ_CP062889
• Organism: Escherichia coli O100:H21 strain Res13-Lact-ER07-18

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IMP41_RS06180
• Protein ID: WP_000911330.1
• Coordinates: 1280710..1281108 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: IMP41_RS06175
• Protein ID: WP_000450524.1
• Coordinates: 1280483..1280710 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP41_RS06150	1276286..1276756	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
IMP41_RS06155	1276756..1277328	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
IMP41_RS06160	1277474..1278352	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
IMP41_RS06165	1278369..1279403	+	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
IMP41_RS06170	1279616..1280329	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
IMP41_RS06175	1280483..1280710	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
IMP41_RS06180	1280710..1281108	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
IMP41_RS06185	1281255..1282118	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
IMP41_RS06190	1282133..1284148	+	2016	WP_000829292.1	tRNA cytosine(34) acetyltransferase TmcA	-
IMP41_RS06195	1284222..1284920	+	699	WP_000679812.1	esterase	-
IMP41_RS06200	1285001..1285201	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T177154 WP_000911330.1 NZ_CP062889:1280710-1281108 [Escherichia coli O100:H21]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T177154 NZ_CP083263:292084-292187 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT177154 WP_000450524.1 NZ_CP062889:1280483-1280710 [Escherichia coli O100:H21]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT177154 NZ_CP083263:c292225-291954 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• AlphaFold DB: A0A829CM33