Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1004063..1004646 Replicon chromosome
Accession NZ_CP062889
Organism Escherichia coli O100:H21 strain Res13-Lact-ER07-18

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag IMP41_RS04815 Protein ID WP_000254745.1
Coordinates 1004311..1004646 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag IMP41_RS04810 Protein ID WP_000581937.1
Coordinates 1004063..1004311 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP41_RS04800 1000402..1001703 + 1302 WP_000046785.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
IMP41_RS04805 1001751..1003985 + 2235 WP_000226815.1 GTP pyrophosphokinase -
IMP41_RS04810 1004063..1004311 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
IMP41_RS04815 1004311..1004646 + 336 WP_000254745.1 endoribonuclease MazF Toxin
IMP41_RS04820 1004717..1005508 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
IMP41_RS04825 1005736..1007373 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
IMP41_RS04830 1007461..1008759 + 1299 WP_000036723.1 phosphopyruvate hydratase -
IMP41_RS04835 1008819..1009157 - 339 Protein_946 YgcG family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T177153 WP_000254745.1 NZ_CP062889:1004311-1004646 [Escherichia coli O100:H21]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T177153 NZ_CP083259:c2573081-2572974 [Staphylococcus aureus]
ATGTTCAATTTATTAATTGACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT177153 WP_000581937.1 NZ_CP062889:1004063-1004311 [Escherichia coli O100:H21]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT177153 NZ_CP083259:2572897-2572957 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References