Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 867728..868382 Replicon chromosome
Accession NZ_CP062889
Organism Escherichia coli O100:H21 strain Res13-Lact-ER07-18

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag IMP41_RS04190 Protein ID WP_000244772.1
Coordinates 867975..868382 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag IMP41_RS04185 Protein ID WP_000354046.1
Coordinates 867728..867994 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP41_RS04160 863016..863759 + 744 WP_000951964.1 SDR family oxidoreductase -
IMP41_RS04165 863816..865249 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
IMP41_RS04170 865294..865605 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
IMP41_RS04175 865769..866428 + 660 WP_000250274.1 hemolysin III family protein -
IMP41_RS04180 866505..867485 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
IMP41_RS04185 867728..867994 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
IMP41_RS04190 867975..868382 + 408 WP_000244772.1 protein YgfX Toxin
IMP41_RS04195 868422..868943 - 522 WP_001055874.1 flavodoxin FldB -
IMP41_RS04200 869055..869951 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
IMP41_RS04205 869976..870686 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IMP41_RS04210 870692..872425 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T177152 WP_000244772.1 NZ_CP062889:867975-868382 [Escherichia coli O100:H21]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T177152 NZ_CP083259:2090985-2091173 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT177152 WP_000354046.1 NZ_CP062889:867728-867994 [Escherichia coli O100:H21]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT177152 NZ_CP083259:2090696-2090956 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATAGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References