Detailed information of TA system TA177146

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 132963..133488
• Replicon: plasmid unnamednovel_0
• Accession: NZ_CP062888
• Organism: Escherichia coli strain Res13-Lact-PEA06-10

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: V0SSI5
• Locus tag: IMP66_RS24830
• Protein ID: WP_001159868.1
• Coordinates: 132963..133268 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1PPD8
• Locus tag: IMP66_RS24835
• Protein ID: WP_000813634.1
• Coordinates: 133270..133488 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP66_RS24805	128795..129439	-	645	WP_029488797.1	replication initiation protein RepE	-
IMP66_RS24810	129467..130372	-	906	Protein_143	IS3 family transposase	-
IMP66_RS24815	130437..131588	+	1152	WP_195686658.1	IS30-like element IS30 family transposase	-
IMP66_RS24820	131601..131903	-	303	Protein_145	transposase	-
IMP66_RS24825	132156..132962	-	807	WP_097423266.1	site-specific integrase	-
IMP66_RS24830	132963..133268	-	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	Toxin
IMP66_RS24835	133270..133488	-	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
IMP66_RS24840	134202..136823	+	2622	WP_023063774.1	ABC transporter	-
IMP66_RS24845	137022..137252	+	231	WP_029488776.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
IMP66_RS24850	137341..137598	+	258	WP_195686660.1	type II toxin-antitoxin system VapC family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..141254	141254
	-	flank	IS/Tn	-	-	137754..138962	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11706.51 Da
• Isoelectric Point: 6.4674

>T177146 WP_001159868.1 NZ_CP062888:c133268-132963 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T177146 NZ_CP083257:c1414791-1414300 [Bifidobacterium longum subsp. longum]
ATGAGGCAGTCAAAGCAGGTGTCGATCTTGTGCGACACCAATATCCTTATTTATTACATGCTCTGTACGCCTCGGTTCAT
CAAGGATGTCATGAAACTGTTCGAGCGATGCGTCGAGCGGGAGGTAAAGCTGTCCTGTGCGTCACTGTCGCTGAAGGACA
TGTTCTACATCACCCCGGAACGATTCAAAGAACGATTCATGAATGACGAATCCATGGTCGTTGCGACCACCGCCAAGCTG
ATATTGCCGGAGGTGGCATGGTCGGGAATCAGCAAACTCAAGGAGCTCATGACAATCGTGCCGATTGGCGCGGAGGAATG
TGAATTGGCGCAGTCGTTGCGGCACGCGCATAACGATTTCGAGGATGACCTTATTATCGCCGCCGCACAACAATCCGGGG
CCACCTATGTGGTGACGTATGACAAAAAACTAGCCAAGCATTTTCCCGAATATTGCAAAACGCCGGCTGAAATCGTGGCG
GCGCTGGCATAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.34 Da
• Isoelectric Point: 4.7405

>AT177146 WP_000813634.1 NZ_CP062888:c133488-133270 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT177146 NZ_CP083257:c1415180-1414788 [Bifidobacterium longum subsp. longum]
ATGACCATGGTCCAAATGAACGTTCGCATTGATGCGAAACTGAAGAACGAGGTCGAAGAAGTCCTCAAGGGCAAAGGCGT
ATCGGTGAGTGACGTAATCCGGTCGCTGTGGTTGTACATCGCCGACCGCAAGGAAGTGCCGGCATTGGAAACGACCGCCG
AAGAGCAAGCCCGCGAGGCGGAAAAGCAGCGCAAGCTGAAACTGATTCGAGAAGGTGCCGGATACGTACACAAGGAACTG
GTCAAGGCCGGACTGATCTCAGAAGACACCGACCTCATGGAAGGCCTTACCTACAAGCAGTTCCGCGATGAGATAGCTGA
CGAACGGCTGAAGGAATACAGGCATCAGTCGGACTATGCGCGTTATTTGGAGAAGGAGCGGAGTCTGTCATGA

Structures

Toxin

• PDB: 4VUB
• PDB: 2VUB
• PDB: 7EPI
• PDB: 7EPG
• PDB: 3VUB
• PDB: 1X75
• PDB: 1VUB
• PDB: 7EPJ
• PDB: 3HPW
• PDB: 3G7Z

Antitoxin

• PDB: 3TCJ
• PDB: 2H3A
• PDB: 2ADN
• PDB: 2ADL
• PDB: 3HPW
• PDB: 2H3C
• PDB: 3G7Z
• AlphaFold DB: A0A829CQY2