Detailed information of TA system TA177140

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 75370..75917
• Replicon: plasmid unnamednovel_0
• Accession: NZ_CP062888
• Organism: Escherichia coli strain Res13-Lact-PEA06-10

Toxin (Protein)

• Gene name: parE
• Uniprot ID: E9LLH3
• Locus tag: IMP66_RS24500
• Protein ID: WP_000969996.1
• Coordinates: 75370..75651 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A2H4TKU7
• Locus tag: IMP66_RS24505
• Protein ID: WP_000079923.1
• Coordinates: 75648..75917 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP66_RS24470	70898..71089	-	192	WP_157895655.1	hypothetical protein	-
IMP66_RS24475	71586..72587	+	1002	WP_029488840.1	DUF2219 family protein	-
IMP66_RS24480	72662..73567	-	906	Protein_77	IS3 family transposase	-
IMP66_RS24485	73577..74727	-	1151	Protein_78	IS30 family transposase	-
IMP66_RS24490	74795..75097	-	303	Protein_79	transposase	-
IMP66_RS24495	75133..75330	-	198	Protein_80	3'-5' exonuclease	-
IMP66_RS24500	75370..75651	-	282	WP_000969996.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IMP66_RS24505	75648..75917	-	270	WP_000079923.1	hypothetical protein	Antitoxin
IMP66_RS24510	76830..77686	-	857	Protein_83	incFII family plasmid replication initiator RepA	-
IMP66_RS24515	77679..77753	-	75	WP_001365705.1	RepA leader peptide Tap	-
IMP66_RS24520	78000..78248	-	249	WP_000083838.1	replication regulatory protein RepA	-
IMP66_RS24525	78532..78738	-	207	WP_032339099.1	type I toxin-antitoxin system Hok family toxin	-
-	78725..78786	+	62	NuclAT_0	-	-
-	78725..78786	+	62	NuclAT_0	-	-
-	78725..78786	+	62	NuclAT_0	-	-
-	78725..78786	+	62	NuclAT_0	-	-
IMP66_RS24530	78961..79344	-	384	Protein_87	hypothetical protein	-
IMP66_RS24535	79462..79908	-	447	WP_032339098.1	thermonuclease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..141254	141254
	-	inside	IScluster/Tn	-	-	72662..86482	13820

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10893.57 Da
• Isoelectric Point: 8.4615

>T177140 WP_000969996.1 NZ_CP062888:c75651-75370 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T177140 NZ_CP083240:c27153-26860 [Ancylobacter polymorphus]
GTGAAGCTTGTATGGTCCGCTTACGCGCTCTCCGACCGTGACGGCATATTCTCTCATATTGAGGCGGATAGTTCTGCCGC
CGCGGCCTCAGTCGATGAGCGGATTGCGGCTGCGGCGCGCCGTCTGCGAGAGTTTCCCGAAAGCGGTCGTCCCGGAAGGA
TCCACGGGACGCGCGAACTGGTGATTGCCGGCACGCCTTATGTCGCGGCCTATGTCGTGACCAAAACGACGGTCCGCATC
CTTCGCGTTCTGCACGGTGCGCAACGGTGGCCGGAGAGCTTGCCCGAGGGTTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10196.52 Da
• Isoelectric Point: 7.2050

>AT177140 WP_000079923.1 NZ_CP062888:c75917-75648 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVEQQHNTWFRDQVAAGRQQLERGDVLPHDMVESSATAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT177140 NZ_CP083240:c27458-27150 [Ancylobacter polymorphus]
ATGGCCGCGAATGCTCTCGTTCAAACCCGCATTGATGCCGAGATCCGGGATCGCGCCTCGGCCGTGCTCGAAAGCATGGG
GCTCACGGTTTCCGATGCCGTGCGCATCCTGCTCACCCGCACCGCCAATGAGGGCGCGCTTCCGCTCGAACTGGTGGCAA
ACAGCGAGGCGCATGACGCCTGGTTCCGGGCTAAGGTATTGGAGGCGCTGAACGACACCCGGCCCGATATCTCCGACGAT
GAGGCCGAGGCACATTTCGCGGAGCGGCGGGCGGCGGCGCGCCGTCGTGCGAGCGACGTCCCAACGTGA

Structures

Toxin

• AlphaFold DB: E9LLH3

Antitoxin

• AlphaFold DB: A0A2H4TKU7