Detailed information of TA system TA177134

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 15173..15774
• Replicon: plasmid unnamed1009
• Accession: NZ_CP062884
• Organism: Escherichia coli strain Res13-Lact-PEA06-10

Toxin (Protein)

• Gene name: doc
• Uniprot ID: U9Z1Q0
• Locus tag: IMP66_RS22910
• Protein ID: WP_001216047.1
• Coordinates: 15394..15774 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: IMP66_RS22905
• Protein ID: WP_001190712.1
• Coordinates: 15173..15394 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP66_RS22870	10448..10636	+	189	WP_000797279.1	hypothetical protein	-
IMP66_RS22875	10638..10816	+	179	Protein_18	hypothetical protein	-
IMP66_RS22880	11150..12160	+	1011	WP_042198315.1	hypothetical protein	-
IMP66_RS22885	12157..12519	+	363	WP_001261543.1	hypothetical protein	-
IMP66_RS22890	13595..13951	-	357	WP_001062545.1	hypothetical protein	-
IMP66_RS22895	13952..14536	-	585	WP_042198310.1	hypothetical protein	-
IMP66_RS22900	14711..15100	+	390	WP_000506730.1	DNA repair protein	-
IMP66_RS22905	15173..15394	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
IMP66_RS22910	15394..15774	+	381	WP_001216047.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
IMP66_RS22915	15779..15958	+	180	WP_000113018.1	hypothetical protein	-
IMP66_RS22920	15986..17029	+	1044	WP_195686630.1	DUF968 domain-containing protein	-
IMP66_RS22925	17118..17570	+	453	WP_001326849.1	late promoter-activating protein	-
IMP66_RS22930	17656..18849	+	1194	WP_047649469.1	hypothetical protein	-
IMP66_RS22935	18849..20333	+	1485	WP_000124159.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul1 / aac(3)-VIa / ant(3'')-Ia	htpB	1..117198	117198

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13574.27 Da
• Isoelectric Point: 5.1514

>T177134 WP_001216047.1 NZ_CP062884:15394-15774 [Escherichia coli]
MRHISPEELVALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T177134 NZ_CP083239:c88326-87901 [Ancylobacter polymorphus]
TTGAATGATCATCCTCGAATCACCAATGTCGTCTCCGAGCCGATGCGCCCGGCCGGCAGCCCTGCGGTGCTGGACTGGCT
CGACCGTCAGGCCGCGTCGACGCTGTTTCTGACCGGCACCAGCCTGTCTGAACTCATGCTCGGCGCGGAAATCCTGCCCG
CCGGCCGCCGGCGCGAGGAACTGCGCGCGGGCCTGGCCGAACTTCTTGATACCTTGTTCGATAGCCGCATCCTGCCGTTC
GACGCGGCCGCCGCGCACGCCTTCGCCCGGCTGGTGGCGCAAGCCCGGAGCGCCGGGCGGGCGATTTCCGTGGCGGACGG
CCAGATCGCCGCCATCGCCACCGCGCATGGTTTCCGCGTCGCCACCCGCGACCGCGCGCCCTTTGAGGCGGCGGGCGTGC
CGGTCATCGACCCCTGGGCGGAATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT177134 WP_001190712.1 NZ_CP062884:15173-15394 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT177134 NZ_CP083239:c88558-88319 [Ancylobacter polymorphus]
ATGTCGGCCGTCACCATTCGCAACCTTCCCGACGAGGTTCACCGTGCCCTCAAGGACCGGGCGGCGCGCCATGGGCGCAG
CACGGAAGCGGAGATCCGCGAAATATTGCAGGCCGCTGTGCGCCCGCCGGAGCGGGTCAAGGTCGGCTCGGCCCTCGCTG
CCTTCGGTCGTCGCTTCAATGGGATCGAGTTCGAGCCCATGCGTGCGAGCGACCCGGCTGAGCCGGCCTCGTTTGAATGA

Structures

Toxin

• AlphaFold DB: U9Z1Q0

Antitoxin

• AlphaFold DB: A0A829CJB6