Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4269841..4270338 Replicon chromosome
Accession NZ_CP062883
Organism Escherichia coli strain Res13-Lact-PEA06-10

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag IMP66_RS20630 Protein ID WP_001173343.1
Coordinates 4269841..4270014 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag IMP66_RS20635 Protein ID WP_000398619.1
Coordinates 4270042..4270338 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP66_RS20620 4266511..4267968 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
IMP66_RS20625 4268205..4269722 + 1518 WP_001295074.1 lysine--tRNA ligase -
IMP66_RS20630 4269841..4270014 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
IMP66_RS20635 4270042..4270338 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
IMP66_RS20640 4270565..4270837 - 273 WP_000405651.1 N-acetyltransferase -
IMP66_RS20645 4270849..4271079 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
IMP66_RS20650 4271260..4272891 + 1632 WP_001216477.1 sensor histidine kinase -
IMP66_RS20655 4272888..4273607 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T177133 WP_001173343.1 NZ_CP062883:c4270014-4269841 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T177133 NZ_CP083182:c102264-101968 [Acinetobacter baumannii]
ATGATTGAAATTAAACGTCTGCCTGAATTTGATGAATGGCTAGATGGTCTTAAAGACAATATGACTCGAATCCGCTTGAA
TCGTCGATTAGATAAGGTCCAACGTGGTAATTGGGGTGACATTAAACCACTCCAAGATGGTGTCTGGGAAATGAGAGAGT
TCTTTGGTGCTGGTTGGCGGATGTACTACATCCAACATGGTGACGTTGTGATCGTGATGCTTGGTGGTGGTGAAAAATCT
ACACAGAAAGATGACATCAAGAAAGCGGTCAAACTATCGAAAACATTGGAGGATTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT177133 WP_000398619.1 NZ_CP062883:c4270338-4270042 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT177133 NZ_CP083182:c101966-101676 [Acinetobacter baumannii]
ATGGTTAAAGTCGCTGATTTACCAAGTTTTGATATGGCTGAGTCACTCAAAACTGAAGAAGATATTGTAATGTACCTCAA
TATGGTTCTTGAAGAAAATGATCCTGCTGAATTAGCGCACGCACTTGGTGTAATTGCTAAAGCAAGAGGTATGACCCAAA
TTGCGAAAGAAGCAGGTATTGGACGAGAAGCCCTTTACAAAGCACTTCGTCAAGACTCAACGCCTCGTTTTGATACGATC
AACCGTGTTGTGAATGCTTTAGGTTTAAAATTGACAGTTCAACATACTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References