Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 18693..19186 Replicon plasmid unnamednovel_3
Accession NZ_CP062881
Organism Escherichia coli O157:H16 strain Res13-Lact-PEA12-26

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag IMP69_RS25400 Protein ID WP_001230709.1
Coordinates 18693..18947 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag IMP69_RS25405 Protein ID WP_000151456.1
Coordinates 18944..19186 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP69_RS25365 13740..14042 - 303 WP_001096541.1 hypothetical protein -
IMP69_RS25370 14132..14791 - 660 WP_016235966.1 AAA family ATPase -
IMP69_RS25375 15343..15666 + 324 WP_016235965.1 hypothetical protein -
IMP69_RS25380 16020..16364 + 345 WP_001146936.1 type II toxin-antitoxin system RelE/ParE family toxin -
IMP69_RS25385 16351..16701 + 351 WP_000127482.1 addiction module antitoxin -
IMP69_RS25390 17535..18050 + 516 WP_001025393.1 J domain-containing protein -
IMP69_RS25395 18154..18696 + 543 WP_016235963.1 hypothetical protein -
IMP69_RS25400 18693..18947 + 255 WP_001230709.1 hypothetical protein Toxin
IMP69_RS25405 18944..19186 + 243 WP_000151456.1 hypothetical protein Antitoxin
IMP69_RS25410 19757..20050 + 294 WP_000673428.1 hypothetical protein -
IMP69_RS25415 20127..20264 + 138 WP_001330659.1 hypothetical protein -
IMP69_RS25420 20355..20642 + 288 WP_000606467.1 hypothetical protein -
IMP69_RS25425 21070..21303 + 234 WP_000093744.1 hypothetical protein -
IMP69_RS25430 21379..21681 + 303 WP_000379302.1 hypothetical protein -
IMP69_RS25435 21992..22669 - 678 WP_000606419.1 hypothetical protein -
IMP69_RS25440 22719..23474 - 756 WP_000797968.1 conjugal transfer protein TraL -
IMP69_RS25445 23492..23893 - 402 WP_000717787.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..44740 44740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(10-243)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T177111 WP_001230709.1 NZ_CP062881:18693-18947 [Escherichia coli O157:H16]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T177111 NZ_CP083144:2167493-2168230 [Bacillus thuringiensis]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
AGAGTTGTACGAGGAGAAGAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACGCCTAATATTTCAGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCCATAAAACGATC
ATTAATTGCATCTAAAGCAAGAGGAGAAATATACACGACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGCGCGAAAATT
GTTGTTTTTGAAGCTGCGAAGGAATTAAATACAAACTTTCGTGGTATTCGCTCACAATTTAGTATTATCGTCCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT177111 WP_000151456.1 NZ_CP062881:18944-19186 [Escherichia coli O157:H16]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT177111 NZ_CP083144:2168343-2168471 [Bacillus thuringiensis]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGATAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References