Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 12190..12797 Replicon plasmid unnamednovel_3
Accession NZ_CP062881
Organism Escherichia coli O157:H16 strain Res13-Lact-PEA12-26

Toxin (Protein)


Gene name doc Uniprot ID B7LIH8
Locus tag IMP69_RS25345 Protein ID WP_000673985.1
Coordinates 12190..12576 (-) Length 129 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID B7LIH9
Locus tag IMP69_RS25350 Protein ID WP_000493532.1
Coordinates 12573..12797 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP69_RS25340 10010..11038 + 1029 WP_077125671.1 replication initiation protein -
IMP69_RS25345 12190..12576 - 387 WP_000673985.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
IMP69_RS25350 12573..12797 - 225 WP_000493532.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
IMP69_RS25355 12977..13258 - 282 WP_001290347.1 membrane protein -
IMP69_RS25360 13420..13704 - 285 WP_000930236.1 hypothetical protein -
IMP69_RS25365 13740..14042 - 303 WP_001096541.1 hypothetical protein -
IMP69_RS25370 14132..14791 - 660 WP_016235966.1 AAA family ATPase -
IMP69_RS25375 15343..15666 + 324 WP_016235965.1 hypothetical protein -
IMP69_RS25380 16020..16364 + 345 WP_001146936.1 type II toxin-antitoxin system RelE/ParE family toxin -
IMP69_RS25385 16351..16701 + 351 WP_000127482.1 addiction module antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..44740 44740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(11-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-52)


Sequences

Toxin        


Download         Length: 129 a.a.        Molecular weight: 14370.62 Da        Isoelectric Point: 7.0732

>T177110 WP_000673985.1 NZ_CP062881:c12576-12190 [Escherichia coli O157:H16]
MKFVSSQEVIEFHDRLISRDGGVAGMPEPGRADAIIHRVLNMYHYEGVTDIMDLAAVYLVAIARGHIFNDANKRTALFVA
QVFLKRNGVHIISSRISFDEMQIIALNAATGEYNWKMVSDHLKAIILN

Download         Length: 387 bp

>T177110 NZ_CP083134:c3192150-3191800 [Bacillus thuringiensis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTAGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8361.39 Da        Isoelectric Point: 5.6713

>AT177110 WP_000493532.1 NZ_CP062881:c12797-12573 [Escherichia coli O157:H16]
MFTCNYSHARDNLASLLELAVDENQPVEIQRRKGGAAVLVDKDAFERYRKAALDAEFAEIMQSHGTAYTELSKR

Download         Length: 225 bp

>AT177110 NZ_CP083134:c3192442-3192155 [Bacillus thuringiensis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTATATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCG
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A086V9Y9

Antitoxin

Source ID Structure
AlphaFold DB A0A086V9Y8

References